• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

ASUN Recombinant antibody

ASUN Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

241586E9

Cat no : 84252-4-RR

Synonyms

INTS13, Integrator complex subunit 13, Germ cell tumor 1, GCT1, C12orf11

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using ASUN Recombinant antibody (84252-4-RR)

    WB analysis using 84252-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84252-4-RR (C12orf11 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ASUN Recombinant antibody (84252-4-RR)

    IF Staining of HeLa using 84252-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ASUN antibody (84252-4-RR, Clone: 241586E9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

Tested Applications

Positive WB detected inHeLa cells, Jurkat cells, MCF-7 cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84252-4-RR is ASUN Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ASUN fusion protein Ag13727
Full Name chromosome 12 open reading frame 11
Calculated Molecular Weight 706 aa, 80 kDa
Observed Molecular Weight70-80 kDa
GenBank Accession NumberBC003081
Gene Symbol ASUN
Gene ID (NCBI) 55726
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Asunder (ASUN) is a conserved protein that has been reported as a critical regulator of dynein localization during Drosophila spermatogenesis (PMID: 19357193). Mammalian and Drosophila ASUN proteins revealed that they are 43% identical and 64% similar. Human ASUN (also known as GCT1 or C12ORF11) promotes dynein recruitment and centrosomal tethering to the nucleus at mitotic entry (PMID: 23097494).

Protocols

Product Specific Protocols
WB protocol for ASUN antibody 84252-4-RRDownload protocol
IF protocol for ASUN antibody 84252-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols