CoraLite® Plus 647-conjugated Beta Catenin Polyclonal antibody

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  IF Staining of MCF-7 using CL647-51067

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite® Plus 647 Beta Catenin antibody (CL647-51067) at dilution of 1:200.

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  FC experiment of MCF-7 using CL647-51067

  1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite® Plus 647 Anti-Human Beta Catenin (CL647-51067) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of HeLa using CL647-51067

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 647 Anti-Human Beta Catenin (CL647-51067) (red), or 0.4 ug x. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• Featured Product
• KD/KO Validated

Beta Catenin Polyclonal Antibody for IF/ICC, FC (Intra)

Cat No. CL647-51067

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

IF/ICC, FC (Intra)

Synonyms

CTNNB, Catenin beta-1, Catenin beta 1, beta-Catenin, B-catenin

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Tested Applications

• Positive IF/ICC detected in: MCF-7 cells
• Positive FC (Intra) detected in: MCF-7 cells, HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-51067 targets Beta Catenin in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: catenin (cadherin-associated protein), beta 1, 88kDa
• Calculated Molecular Weight: 781 aa, 86 kDa
• Observed Molecular Weight: 92 kDa
• GenBank Accession Number: BC058926
• Gene Symbol: Beta Catenin
• Gene ID (NCBI): 1499
• ENSEMBL Gene ID: ENSG00000168036
• RRID: AB_2920240
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P35222
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

β-Catenin, also known as CTNNB1, is an evolutionarily conserved, multifunctional intracellular protein. β-Catenin is a 92-kDa protein, originally identified in cell adherens junctions (AJs) where it functions to bridge the cytoplasmic domain of cadherins to a-catenin and the actin cytoskeleton. Besides its essential role in the AJs, β-catenin is also a key downstream component of the canonical Wnt pathway that plays diverse and critical roles in embryonic development and adult tissue homeostasis. The Wnt/β-catenin pathway is also involved in the activation of other intracellular messengers such as calcium fluxes, JNK, and SRC kinases. Deregulation of β-catenin activity is associated with multiple diseases including cancers. (PMID: 22617422; 18334222)