BTN2A1 Recombinant antibody

BTN2A1 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241540F10

Cat no : 84550-1-RR

Synonyms

BTF1, BT2.1, 241540F10

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Validation Data Gallery

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  WB analysis of HeLa using 84550-1-RR

  HeLa cells were subjected to SDS PAGE followed by western blot with 84550-1-RR (BTN2A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 84550-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84550-1-RR (BTN2A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  Affinity and Kinetic Characterization of 84550-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84550-1-RR against Human BTN2A1 were performed. The affinity constant is 35.9 nM.

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Tested Applications

• Positive WB detected in: HeLa cells, THP-1 cells, MCF-7 cells, NCCIT cells, LNCaP cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84550-1-RR is BTN2A1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: butyrophilin, subfamily 2, member A1
• Calculated Molecular Weight: 60 kDa
• Observed Molecular Weight: 65 kDa
• GenBank Accession Number: BC016661
• Gene Symbol: BTN2A1
• Gene ID (NCBI): 11120
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BTN2A1, also named as BT2.1, BTF1 is a 527 amino acid protein, which belongs to the immunoglobulin superfamily. BTN2A1 is highly expressed in brain, bone marrow, small intestine, muscle, spleen and pancreas and moderate expression was seen in lung, liver and kidney. The calcualted molecular weight of BTN2A1 is 59 kDa, but glycosylation of BTN2A1 is about 65-69 kDa. (PMID: 17785817)