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BTN2A1 Recombinant antibody, PBS Only

BTN2A1 Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241540F10

Cat no : 84550-1-PBS

Synonyms

BTF1, BT2.1, 241540F10

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using BTN2A1 Recombinant antibody (84550-1-RR)

    WB analysis of HeLa using 84550-1-RR (same clone as 84550-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 84550-1-RR (BTN2A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84550-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using BTN2A1 Recombinant antibody (84550-1-RR)

    WB analysis using 84550-1-RR (same clone as 84550-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84550-1-RR (BTN2A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84550-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84550-1-RR

    Affinity and Kinetic Characterization of 84550-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84550-1-RR against Human BTN2A1 were performed. The affinity constant is 35.9 nM.

Product Information

The immunogen of 84550-1-PBS is BTN2A1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein
Full Name butyrophilin, subfamily 2, member A1
Calculated Molecular Weight 60 kDa
Observed Molecular Weight65 kDa
GenBank Accession NumberBC016661
Gene Symbol BTN2A1
Gene ID (NCBI) 11120
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

BTN2A1, also named as BT2.1, BTF1 is a 527 amino acid protein, which belongs to the immunoglobulin superfamily. BTN2A1 is highly expressed in brain, bone marrow, small intestine, muscle, spleen and pancreas and moderate expression was seen in lung, liver and kidney. The calcualted molecular weight of BTN2A1 is 59 kDa, but glycosylation of BTN2A1 is about 65-69 kDa. (PMID: 17785817)