BSAP,PAX5 Monoclonal antibody, PBS Only (Capture)

• Featured Product
• KD/KO Validated

BSAP,PAX5 Monoclonal Antibody for WB, IHC, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2H2D6

Cat no : 60349-1-PBS

Synonyms

PAX5, PAX 5, Paired box protein Pax-5, Paired box protein Pax 5, paired box 5

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Validation Data Gallery

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  WB analysis of Ramos using 60349-1-Ig (same clone as 60349-1-PBS)

  Ramos cells were subjected to SDS PAGE followed by western blot with 60349-1-Ig (BSAP,PAX5 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60349-1-PBS in a different storage buffer formulation.

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  WB analysis of Daudi using 60349-1-Ig (same clone as 60349-1-PBS)

  Daudi cells were subjected to SDS PAGE followed by western blot with 60349-1-Ig (BSAP,PAX5 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60349-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Raji using 60349-1-Ig (same clone as 60349-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 60349-1-Ig (BSAP,PAX5 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60349-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 60349-1-Ig (same clone as 60349-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60349-1-Ig (BSAP,PAX5 Antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60349-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 60349-1-Ig (same clone as 60349-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60349-1-Ig (BSAP,PAX5 Antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60349-1-PBS in a different storage buffer formulation.

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  FC experiment of Ramos using 60349-1-Ig (same clone as 60349-1-PBS)

  1X10^6 Ramos cells were intracellularly stained with 0.4 ug Anti-Human BSAP,PAX5 (60349-1-Ig, Clone:2H2D6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 60349-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50228-2

  Cytometric bead array standard curve of MP50228-2, BSAP,PAX5 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60349-1-PBS. Detection antibody: 60349-3-PBS. Standard:Ag16023. Range: 3.125-100 ng/mL.

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Product Information

60349-1-PBS targets BSAP,PAX5 as part of a matched antibody pair:

MP50228-2: 60349-1-PBS capture and 60349-3-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: BSAP,PAX5 fusion protein Ag16023
• Full Name: paired box 5
• Calculated Molecular Weight: 391 aa, 42 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: BC156927
• Gene Symbol: PAX5
• Gene ID (NCBI): 5079
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

PAX5, also named B-cell specific activator protein (BSAP), is a member of the highly conserved paired box (PAX)-domain family of transcription factors. PAX5 is essential in normal B-cell lymphopoiesis. It is expressed in pro-B, pre-B and mature B lymphocytes but not in plasma cells. PAX5 is a common target in leukemogenesis of B-ALL ( B-lineage acute lymphoblastic leukemia) along with CDKN2A. Owing to its frequent deletion in B-ALL, PAX5 could be used as one of the molecular markers in diagnosis and monitoring of the disease. This antibody is specific to PAX5. It will not cross react with other PAX members.