CoraLite® Plus 647-conjugated BOULE Polyclonal antibody

BOULE Polyclonal Antibody for IF-P

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF-P

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Cat no : CL647-13720

Synonyms

BOLL, Protein boule-like

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Validation Data Gallery

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  IF Staining of mouse testis using CL647-13720

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using CoraLite® Plus 647 BOULE antibody (CL647-13720) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse testis using CL647-13720

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using CoraLite® Plus 647 BOULE antibody (CL647-13720) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IF-P detected in: mouse testis tissue

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-13720 targets BOULE in IF-P applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BOULE fusion protein Ag4662
• Full Name: bol, boule-like (Drosophila)
• Calculated Molecular Weight: 283 aa, 31 kDa
• Observed Molecular Weight: 38-42 kDa
• GenBank Accession Number: BC033674
• Gene Symbol: BOULE
• Gene ID (NCBI): 66037
• RRID: AB_2920223
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage.