BOULE Polyclonal antibody

BOULE Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, IP, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 13720-1-AP

Synonyms

BOLL, Protein boule-like

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Validation Data Gallery

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  WB analysis using 13720-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13720-1-AP (BOULE antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 13720-1-AP

  IP result of anti-BOULE (IP:13720-1-AP, 4ug; Detection:13720-1-AP 1:1000) with mouse testis tissue lysate 4000ug.

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  IHC staining of mouse testis using 13720-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13720-1-AP (BOULE antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse ovary using 13720-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 13720-1-AP (BOULE antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 13720-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13720-1-AP (BOULE antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse ovary using 13720-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 13720-1-AP (BOULE antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse testis using 13720-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using BOULE antibody (13720-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse testis using 13720-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using BOULE antibody (13720-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse testis tissue, rat testis tissue
• Positive IP detected in: mouse testis tissue
• Positive IHC detected in: mouse testis tissue, mouse ovary tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

13720-1-AP targets BOULE in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BOULE fusion protein Ag4662
• Full Name: bol, boule-like (Drosophila)
• Calculated Molecular Weight: 283 aa, 31 kDa
• Observed Molecular Weight: 38-42 kDa
• GenBank Accession Number: BC033674
• Gene Symbol: BOULE
• Gene ID (NCBI): 66037
• RRID: AB_2290617
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BOULE, also called BOLL, belongs to the RRM DAZ family. BOULE has been identified as being involved in regulating the development and differentiation of germ cells and playing an essential role in infertility (PMID: 31683986). BOULE is specifically expressed in testis, but not in early embryos, primordial germ cells, and spermatogonial cells. It is first expressed in the cytoplasm of spermatocytes and then persists through meiosis. BOULE has 5 isoforms with the molecular mass of 19 and 31-38 kDa. This antibody detects two bands in testis, the lower band of ~40 kDa in size corresponds to the predicted endogenous BOULE protein, while the upper band may be an isoform or posttranslationally modified version of the BOULE protein (PMID: 27632217).

Publications

Species	Application	Title
human	IHC	Andrologia; ATF6 deficiency damages the development of spermatogenesis in male Atf6 knockout mice.; Authors - Ru Yu