CoraLite®594-conjugated BLNK Monoclonal antibody

BLNK Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1A1A1

Cat no : CL594-66953

Synonyms

SLP65, Cytoplasmic adapter protein, B-cell linker protein, B-cell adapter containing a Src homology 2 domain protein, B-cell adapter containing a SH2 domain protein

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Validation Data Gallery

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  IF Staining of NIH/3T3 using CL594-66953

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using CoraLite®594 BLNK antibody (CL594-66953, Clone: 1A1A1 ) at dilution of 1:200.

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  FC experiment of Daudi using CL594-66953

  1X10^6 Daudi cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human BLNK (CL594-66953, Clone:1A1A1) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF/ICC detected in: NIH/3T3 cells
• Positive FC (Intra) detected in: Daudi cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66953 targets BLNK in IF/ICC, FC (Intra) applications and shows reactivity with human, pig samples.

• Tested Reactivity: human, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: BLNK fusion protein Ag28673
• Full Name: B-cell linker
• Calculated Molecular Weight: 50 kDa
• Observed Molecular Weight: 65-70 kDa
• GenBank Accession Number: BC018906
• Gene Symbol: BLNK
• Gene ID (NCBI): 29760
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

BLNK (also known as SLP-65 or BASH) is an important adaptor protein selectively expressed in B-lineage cells. BLNK bridges B cell receptor-associated kinase activation with downstream signaling pathways, thereby affecting various biological functions. BLNK has been shown to be necessary for BCR-mediated Ca2+ mobilization, for the activation of mitogen-activated protein kinases such as ERK, JNK, and p38 in a chicken B cell line DT40, and for activation of transcription factors such as NF-AT and NF-κB in human or mouse B cells. BLNK plays a crucial role in pre-BCR-dependent progression of B cell development, BCR-mediated B cell survival, activation, proliferation, and T-independent immune responses.