BCL10 Monoclonal antibody, PBS Only

BCL10 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2E8G5

Cat no : 66556-1-PBS

Synonyms

c E10, Bcl-10, Bcl 10, B-cell lymphoma/leukemia 10, B-cell CLL/lymphoma 10

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Validation Data Gallery

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  WB analysis using 66556-1-Ig (same clone as 66556-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66556-1-Ig (BCL10 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 66556-1-PBS in a different storage buffer formulation.

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  WB analysis of Raji using 66556-1-Ig (same clone as 66556-1-PBS)

  Raji, K-562, and HL-60 cells were subjected to SDS PAGE followed by western blot with 66556-1-Ig (BCL10 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66556-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66556-1-Ig (same clone as 66556-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66556-1-Ig (BCL10 antibody) at dilution of 1:700 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66556-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66556-1-Ig (same clone as 66556-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66556-1-Ig (BCL10 antibody) at dilution of 1:700 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66556-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 66556-1-Ig (same clone as 66556-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66556-1-Ig (BCL10 antibody) at dilution of 1:700 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66556-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lymphoma using 66556-1-Ig (same clone as 66556-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66556-1-Ig (BCL10 antibody) at dilution of 1:700 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66556-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66556-1-Ig (same clone as 66556-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using BCL10 antibody (66556-1-Ig, Clone: 2E8G5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66556-1-PBS in a different storage buffer formulation.

• 

  FC experiment of Jurkat using 66556-1-Ig (same clone as 66556-1-PBS)

  1x10^6 Jurkat cells were intracellularly stained with 0.4 ug BCL10 Monoclonal antibody (66556-1-Ig, Clone:2E8G5) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66556-1-PBS in a different storage buffer formulation.

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Product Information

66556-1-PBS targets BCL10 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: BCL10 fusion protein Ag12162
• Full Name: B-cell CLL/lymphoma 10
• Calculated Molecular Weight: 233 aa, 26 kDa
• Observed Molecular Weight: 30-32 kDa
• GenBank Accession Number: BC053617
• Gene Symbol: BCL10
• Gene ID (NCBI): 8915
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.