CoraLite®594-conjugated BBS5 Monoclonal antibody

BBS5 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

IF-P

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

4G8E12

Cat no : CL594-66136

Synonyms

Bardet Biedl syndrome 5, BBS5

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Validation Data Gallery

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  IF Staining of human kidney using CL594-66136

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using CoraLite®594 BBS5 antibody (CL594-66136, Clone: 4G8E12 ) at dilution of 1:100.

• 

  IF Staining of human kidney using CL594-66136

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using CoraLite®594 BBS5 antibody (CL594-66136, Clone: 4G8E12 ) at dilution of 1:100.

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Tested Applications

• Positive IF-P detected in: human kidney tissue

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL594-66136 is BBS5 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: BBS5 fusion protein Ag6844
• Full Name: Bardet-Biedl syndrome 5
• Calculated Molecular Weight: 39 kDa
• GenBank Accession Number: BC044593
• Gene Symbol: BBS5
• Gene ID (NCBI): 129880
• RRID: AB_2919953
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage.

Background Information

BBS5 encodes a protein that has been directly linked to Bardet-Biedl syndrome. Bardet-Biedl syndrome (BBS) is an autosomal recessive condition characterised by rod-cone dystrophy, postaxial polydactyly, central obesity, mental retardation, hypogonadism, and renal dysfunction. Other associated clinical findings in BBS patients include diabetes, hypertension and congenital heart defects. BBS expression varies both within and between families and diagnosis is often difficult. Experimentation in non-human eukaryotes suggests that BBS5 is expressed in ciliated cells and that it is required for the formation of cilia. Alternate transcriptional splice variants have been observed but have not been fully characterized.