BBS5 Monoclonal antibody

BBS5 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

4G8E12

Cat no : 66136-1-Ig

Synonyms

Bardet Biedl syndrome 5, BBS5

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Validation Data Gallery

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  WB analysis of human brain using 66136-1-Ig

  human brain tissue were subjected to SDS PAGE followed by western blot with 66136-1-Ig (BBS5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse kidney using 66136-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66136-1-Ig (BBS5 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 66136-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66136-1-Ig (BBS5 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 66136-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66136-1-Ig (BBS5 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of mouse testis using 66136-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66136-1-Ig (BBS5 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: human brain tissue
• Positive IHC detected in: mouse kidney tissue, mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 66136-1-Ig is BBS5 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: BBS5 fusion protein Ag6844
• Full Name: Bardet-Biedl syndrome 5
• Calculated Molecular Weight: 39 kDa
• Observed Molecular Weight: 39 kDa
• GenBank Accession Number: BC044593
• Gene Symbol: BBS5
• Gene ID (NCBI): 129880
• RRID: AB_2881535
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BBS5 encodes a protein that has been directly linked to Bardet-Biedl syndrome. Bardet-Biedl syndrome (BBS) is an autosomal recessive condition characterised by rod-cone dystrophy, postaxial polydactyly, central obesity, mental retardation, hypogonadism, and renal dysfunction. Other associated clinical findings in BBS patients include diabetes, hypertension and congenital heart defects. BBS expression varies both within and between families and diagnosis is often difficult. Experimentation in non-human eukaryotes suggests that BBS5 is expressed in ciliated cells and that it is required for the formation of cilia. Alternate transcriptional splice variants have been observed but have not been fully characterized.