BAZ2A Recombinant antibody, PBS Only

BAZ2A Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230205B8

Cat no : 82910-2-PBS

Synonyms

WALp3, TTF I interacting protein 5, TIP5, KIAA0314, hWALp3

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Validation Data Gallery

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  WB analysis using 82910-2-RR (same clone as 82910-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82910-2-RR (BAZ2A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82910-2-PBS in a different storage buffer formulation.

• 

  IF Staining of A549 using 82910-2-RR (same clone as 82910-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using BAZ2A antibody (82910-2-RR, Clone: 230205B8 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82910-2-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 82910-2-RR (same clone as 82910-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using BAZ2A antibody (82910-2-RR, Clone: 230205B8 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82910-2-PBS in a different storage buffer formulation.

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  FC experiment of U-2 OS using 82910-2-RR (same clone as 82910-2-PBS)

  (blue)1x10^6 U-2 OS were intracellularly stained with 0.25 ug Anti-Human BAZ2A (82910-2-RR, Clone:230205B8) and Alexa Fluor™ Plus 647-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:166(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82910-2-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82910-2-RR

  Biolayer interferometry (BLl) kinetic assays of 82910-2-RR against Human BAZ2A were performed. The affinity constant is 1.46 nM.

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Product Information

82910-2-PBS targets BAZ2A in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: BAZ2A fusion protein Ag33963
• Full Name: bromodomain adjacent to zinc finger domain, 2A
• Calculated Molecular Weight: 211 kDa
• Observed Molecular Weight: 250-270 kDa
• GenBank Accession Number: NM_013449.4
• Gene Symbol: BAZ2A
• Gene ID (NCBI): 11176
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

BAZ2A (bromodomain adjacent to zinc finger domain 2A), also known as TIP5. It is expected to be located in nucleus, and the protein is expressed at moderate levels in most tissues analyzed, including heart, brain, placenta, lung, skeletal muscle, kidney and pancreas. BAZ2A is an epigenetic regulator affecting transcription of ribosomal RNA, it is also a regulatory subunit of the ATP-dependent NoRC-1 and NoRC-5 ISWI chromatin remodeling complexes, which form ordered nucleosome arrays on chromatin and facilitate access to DNA during DNA-templated processes such as DNA replication, transcription, and repair (PubMed:28801535). The calculated molecular weight of BAZ2A is 210 kDa.