CoraLite® Plus 488-conjugated BACE1 Polyclonal antibody

• Featured Product
• KD/KO Validated

BACE1 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, zebrafish, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-12807

Synonyms

Asp 2, ASP2, Aspartyl protease 2, BACE, BACE1, Beta secretase 1, FLJ90568, HSPC104, KIAA1149, Memapsin 2

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Validation Data Gallery

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  IF Staining of Neuro-2a using CL488-12807

  Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using CoraLite® Plus 488 BACE1 antibody (CL488-12807) at dilution of 1:100.

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Tested Applications

• Positive IF/ICC detected in: Neuro-2a cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-12807 targets BACE1 in IF/ICC applications and shows reactivity with human, mouse, zebrafish, rat samples.

• Tested Reactivity: human, mouse, zebrafish, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BACE1 fusion protein Ag3559
• Full Name: beta-site APP-cleaving enzyme 1
• Calculated Molecular Weight: 501 aa, 56 kDa
• Observed Molecular Weight: 70-75 kDa, 42-56 kDa
• GenBank Accession Number: BC036084
• Gene Symbol: BACE1
• Gene ID (NCBI): 23621
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

beta-secretase, encoded by BACE1(beta-stie amyloid beta A4 precursor protein-cleavage enzyem1) gene, is a neuron-specific membrane-associated protease that participate in the two step proteolytic processing of the APP. Cleaves at the N-terminus of the A-beta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase. It has a high expression in the brain and pancreas. This protein has 6 isoforms produced by alternative splicing. BACE1 can be detected with different molecular weight: 42-56kD (prozymogen and isoforms), 65-75 (glycosylated forms) and 160kDa (dimer) (PMID:22741101; 21795680).