CoraLite® Plus 647 Anti-Human B7-H3/CD276 Rabbit Recombinant Antibody

B7-H3/CD276 Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

230305A10

Cat no : CL647-98017

Synonyms

CD276, B7-H3, B7H3, B7 homolog 3, B7 H3

---

Validation Data Gallery

• 

  FC experiment of human monocyte-derived immature dendritic cells using CL647-98017

  1x10^6 human monocyte-derived immature dendritic cells were surface stained with 0.25 ug Coralite® Plus 647 B7-H3/Cd276 Recombinant Antibody (CL647-98017, Clone: 230305A10) (red), or 0.25 ug Coralite® Plus 647 Isotype Control (blue). Cells were not fixed.

---

Tested Applications

• Positive FC detected in: human monocyte-derived immature dendritic cells

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL647-98017 is B7-H3/CD276 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: CD276 molecule
• Calculated Molecular Weight: 57 kDa
• GenBank Accession Number: NM_001024736
• Gene Symbol: CD276
• Gene ID (NCBI): 80381
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light.

Background Information

B7-H3 (CD276) is a type I transmembrane protein expressed on many tissues and cell types. B7-H3 is a 100-kDa glycoprotein that belongs to the B7 immunoregulatory family and participates in the regulation of T-cell-mediated immune response probably by functioning as both a T cell costimulator and coinhibitor (PMID: 25567370; 20696859). Overexpressed on a wide range of human solid cancers, B7-H3 has been implicated in cancer progression and metastasis and becomes an attractive target for cancer immunotherapy (PMID: 27208063).