Anti-Human B7-H3/CD276 Rabbit Recombinant Antibody

B7-H3/CD276 Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

230305A10

Cat no : 98017-1-RR

Synonyms

CD276, B7-H3, B7H3, B7 homolog 3, B7 H3

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Validation Data Gallery

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  FC experiment of human monocyte-derived immature dendritic cells using 98017-1-RR

  1x10^6 human monocyte-derived immature dendritic cells were surface stained with 0.25 ug Anti-Human B7-H3/CD276 Rabbit Recombinant Antibody (98017-1-RR, Clone: 230305A10) (red) or 0.25 ug Isotype Control(blue), and PE-conjugated Goat Anti-Rabbit IgG. Cells were not fixed.

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  Affinity and Kinetic Characterization of 98017-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98017-1-RR against Human B7-H3/CD276 were performed. The affinity constant is 0.203 nM.

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Tested Applications

• Positive FC detected in: human monocyte-derived immature dendritic cells

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 98017-1-RR is B7-H3/CD276 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: CD276 molecule
• Calculated Molecular Weight: 57 kDa
• GenBank Accession Number: NM_001024736
• Gene Symbol: CD276
• Gene ID (NCBI): 80381
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

B7-H3 (CD276) is a type I transmembrane protein expressed on many tissues and cell types. B7-H3 is a 100-kDa glycoprotein that belongs to the B7 immunoregulatory family and participates in the regulation of T-cell-mediated immune response probably by functioning as both a T cell costimulator and coinhibitor (PMID: 25567370; 20696859). Overexpressed on a wide range of human solid cancers, B7-H3 has been implicated in cancer progression and metastasis and becomes an attractive target for cancer immunotherapy (PMID: 27208063).