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Anti-Human B7-H3/CD276 Rabbit Recombinant Antibody, PBS Only

B7-H3/CD276 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

230305A10

Cat no : 98017-1-PBS

Synonyms

CD276, B7-H3, B7H3, B7 homolog 3, B7 H3

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human monocyte-derived immature dendritic cells using Anti-Human B7-H3/CD276 Rabbit Recombinant Antibody (98017-1-RR)

    FC experiment of human monocyte-derived immature dendritic cells using 98017-1-RR (same clone as 98017-1-PBS)

    1x10^6 human monocyte-derived immature dendritic cells were surface stained with 0.25 ug Anti-Human B7-H3/CD276 Rabbit Recombinant Antibody (98017-1-RR, Clone: 230305A10) (red) or 0.25 ug Isotype Control(blue), and PE-conjugated Goat Anti-Rabbit IgG. Cells were not fixed. This data was developed using the same antibody clone with 98017-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 98017-1-RR

    Affinity and Kinetic Characterization of 98017-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98017-1-RR against Human B7-H3/CD276 were performed. The affinity constant is 0.203 nM.

Product Information

The immunogen of 98017-1-PBS is B7-H3/CD276 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein
Full Name CD276 molecule
Calculated Molecular Weight57 kDa
GenBank Accession NumberNM_001024736
Gene Symbol CD276
Gene ID (NCBI) 80381
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

B7-H3 (CD276) is a type I transmembrane protein expressed on many tissues and cell types. B7-H3 is a 100-kDa glycoprotein that belongs to the B7 immunoregulatory family and participates in the regulation of T-cell-mediated immune response probably by functioning as both a T cell costimulator and coinhibitor (PMID: 25567370; 20696859). Overexpressed on a wide range of human solid cancers, B7-H3 has been implicated in cancer progression and metastasis and becomes an attractive target for cancer immunotherapy (PMID: 27208063).