CoraLite® Plus 488-conjugated Aquaporin 4 Polyclonal antibody

• Featured Product
• KD/KO Validated

Aquaporin 4 Polyclonal Antibody for IF-P

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Publications

1

Reviews (1)

Cat no : CL488-16473

Synonyms

AQP 4, AQP4, aquaporin 4, HMIWC2, MIWC, WCH4

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Validation Data Gallery

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  IF Staining of mouse brain using CL488-16473

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 Aquaporin 4 antibody (CL488-16473) at dilution of 1:200.

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Tested Applications

• Positive IF-P detected in: mouse brain tissue

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

The immunogen of CL488-16473 is Aquaporin 4 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Aquaporin 4 fusion protein Ag9561
• Full Name: Aquaporin 4
• Calculated Molecular Weight: 323 aa, 35 kDa
• Observed Molecular Weight: 35-37 kDa, 32-34 kDa
• GenBank Accession Number: BC022286
• Gene Symbol: Aquaporin 4
• Gene ID (NCBI): 361
• RRID: AB_2919135
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Aquaporins are specialized water transport channels in plasma membranes of water-permeable tissues. Aquaporin-4 (AQP4) is the most abundant water channel in the human central nervous system and is important to fluid movements in brain. Aquaporin-4 exists as two isoforms, a long (M1) isoform with translation initiation at Met-1, and a shorter (M23) isoform with translation initiation at Met-23, with molecular weights around 35-37 kDa and 32-34 kDa, respectively.

Publications

Species	Application	Title
mouse	IF	Cell Mol Life Sci; Short-chain fatty acids suppresses astrocyte activation by amplifying Trp-AhR-AQP4 signaling in experimental autoimmune encephalomyelitis mice; Authors - Xiuli Lin

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tinne (Verified Customer) (06-27-2023)

I found this antibody to work really cell in IF!

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mixed culture of human neurons and astrocytes

CL Plus 488 Aquaporin 4 Antibody Immunofluorescence validation (1:500 dilution) in Mixed culture of human neurons and astrocytes (Cat no:CL488-16473)