CoraLite® Plus 488-conjugated Apolipoprotein AI Monoclonal antibody

Apolipoprotein AI Monoclonal Antibody for IF/ICC
Cat No. CL488-66206
Clone No.1C9G5

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse

Applications

IF/ICC

Apo AI, ApoA I, APOA1, apolipoprotein A I, Apolipoprotein A1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive IF/ICC detected inHepG2 cells, L02 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66206 targets Apolipoprotein AI in IF/ICC applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Apolipoprotein AI fusion protein Ag21920 Predict reactive species
Full Name apolipoprotein A-I
Calculated Molecular Weight 31 kDa
Observed Molecular Weight 26-28 kDa
GenBank Accession NumberBC005380
Gene Symbol APOA1
Gene ID (NCBI) 335
RRIDAB_2883284
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP02647
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ApoA1 is a major protein component of high density lipoproteins (HDL) which is associated with reversed cholesterol transport, lipid/cholesterol binding, lecithin/cholesterol acyltransferase (LCAT) activation and specific receptors binding. It is synthesized in the liver and small intestine. Defects of ApoA1 cause low HDL level and systemic non-neuropathic amyloidosis. Serum concentration of ApoA1 is inversely related to the risk of developing atherosclerosis. This antibody was generated against the C-terminal region of human ApoA1.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 Apolipoprotein AI antibody CL488-66206Download protocol
Standard Protocols
Click here to view our Standard Protocols
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