Apolipoprotein AI Monoclonal antibody

Apolipoprotein AI Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

Publications

9

CloneNo.

1C9G5

Cat no : 66206-1-Ig

Synonyms

APOA1, apolipoprotein A I, Apo-AI, ApoAI, ApoA I

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Validation Data Gallery

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  WB analysis of human plasma using 66206-1-Ig

  human plasma were subjected to SDS PAGE followed by western blot with 66206-1-Ig (APOA1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 66206-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66206-1-Ig (APOA1 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 66206-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66206-1-Ig (APOA1 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 66206-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66206-1-Ig (APOA1 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 66206-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66206-1-Ig (APOA1 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 66206-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 66206-1-Ig (APOA1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L).

• 

  FC experiment of HepG2 using 66206-1-Ig

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human Apolipoprotein AI (66206-1-Ig, Clone:1C9G5) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: human plasma tissue
• Positive IHC detected in: human liver cancer tissue, mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 9 publications below

Product Information

66206-1-Ig targets Apolipoprotein AI in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: Apolipoprotein AI fusion protein Ag21920
• Full Name: apolipoprotein A-I
• Calculated Molecular Weight: 31 kDa
• Observed Molecular Weight: 26-28 kDa
• GenBank Accession Number: BC005380
• Gene Symbol: APOA1
• Gene ID (NCBI): 335
• RRID: AB_2881597
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ApoA1 is a major protein component of high density lipoproteins (HDL) which is associated with reversed cholesterol transport, lipid/cholesterol binding, lecithin/cholesterol acyltransferase (LCAT) activation and specific receptors binding. It is synthesized in the liver and small intestine. Defects of ApoA1 cause low HDL level and systemic non-neuropathic amyloidosis. Serum concentration of ApoA1 is inversely related to the risk of developing atherosclerosis. This antibody was generated against the C-terminal region of human ApoA1.

Publications

Species	Application	Title
human	WB	J Extracell Vesicles; Optimized AF4 combined with density cushion ultracentrifugation enables profiling of high-purity human blood extracellular vesicles; Authors - Liqiao Hu
human	WB	Elife; A critical role of VMP1 in lipoprotein secretion.; Authors - Hideaki Morishita
mouse	WB	Mol Cell Proteomics; Differential proteomic analysis of gender-dependent hepatic tumorigenesis in Hras12V transgenic mice.; Authors - Zhuona Rong
human,rat	WB	Peptides; Neuropeptide Y promotes hepatic apolipoprotein A1 synthesis and secretion through neuropeptide Y Y5 receptor.; Authors - Bingyang Liu
mouse	WB	JCI Insight; Aldehyde dehydrogenase 2 and PARP1 interaction modulates hepatic HDL biogenesis by LXRα-mediated ABCA1 expression.; Authors - Luxiao Li
mouse	WB	Front Nutr; Fenofibrate enhances lipid deposition via modulating PPARγ, SREBP-1c, and gut microbiota in ob/ob mice fed a high-fat diet; Authors - Ying Zhang

Related Products

Flow Cytometry Perm Buffer (10X)

PF00011-C