Annexin A11 Monoclonal antibody, PBS Only

Annexin A11 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, rabbit, pig

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1A3C4

Cat no : 68089-1-PBS

Synonyms

56 kDa autoantigen, Annexin 11, annexin A11, Annexin XI, ANX11, ANXA11, CAP 50, CAP50

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Validation Data Gallery

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  WB analysis using 68089-1-Ig (same clone as 68089-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68089-1-Ig (Annexin A11 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68089-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 68089-1-Ig (same clone as 68089-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68089-1-Ig (Annexin A11 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68089-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 68089-1-Ig (same clone as 68089-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68089-1-Ig (Annexin A11 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68089-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 68089-1-Ig (same clone as 68089-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 68089-1-Ig (Annexin A11 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68089-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 68089-1-Ig (same clone as 68089-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 68089-1-Ig (Annexin A11 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68089-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 68089-1-Ig (same clone as 68089-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Annexin A11 antibody (68089-1-Ig, Clone: 1A3C4 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68089-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68089-1-PBS targets Annexin A11 in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse, rat, rabbit, pig samples.

• Tested Reactivity: Human, mouse, rat, rabbit, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Annexin A11 fusion protein Ag31839
• Full Name: annexin A11
• Calculated Molecular Weight: 56 kDa
• GenBank Accession Number: BC007564
• Gene Symbol: ANXA11
• Gene ID (NCBI): 311
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Annexin A11 (Anxa11) is one member of annexins that are Ca2+-regulated phospholipid-binding proteins. Annexin A11 plays an important role in cell division, differentiation, apoptosis, vesicle trafficking and Ca2+ signaling. Annexin A11 dysregulation is involved in the progression, drug-resistance, recurrence of systemic autoimmune disease and cancer.