Annexin A11 Monoclonal antibody

Annexin A11 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

Publications

2

CloneNo.

1A3C4

Cat no : 68089-1-Ig

Synonyms

ANXA11, ANX11, Annexin-11, Annexin XI, Annexin 11

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Validation Data Gallery

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  WB analysis using 68089-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68089-1-Ig (Annexin A11 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  IHC staining of human lung cancer using 68089-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68089-1-Ig (Annexin A11 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 68089-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68089-1-Ig (Annexin A11 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 68089-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 68089-1-Ig (Annexin A11 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 68089-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 68089-1-Ig (Annexin A11 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 68089-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Annexin A11 antibody (68089-1-Ig, Clone: 1A3C4 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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  FC experiment of HEK-293T using 68089-1-Ig

  1X10^6 HEK-293T cells were intracellularly stained with 0.4 ug Anti-Human Annexin A11 (68089-1-Ig, Clone:1A3C4) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  FC experiment of HeLa using 68089-1-Ig

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Annexin A11 (68089-1-Ig, Clone:1A3C4) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: A431 cells, A549 cells, HeLa cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, pig heart tissue, rabbit heart
• Positive IHC detected in: human lung cancer tissue, human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: HEK-293T cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

68089-1-Ig targets Annexin A11 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

• Tested Reactivity: human, mouse, rat, pig, rabbit
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Annexin A11 fusion protein Ag31839
• Full Name: annexin A11
• Calculated Molecular Weight: 56 kDa
• Observed Molecular Weight: 56 kDa
• GenBank Accession Number: BC007564
• Gene Symbol: Annexin A11
• Gene ID (NCBI): 311
• RRID: AB_2918826
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Annexin A11 (Anxa11) is one member of annexins that are Ca2+-regulated phospholipid-binding proteins. Annexin A11 plays an important role in cell division, differentiation, apoptosis, vesicle trafficking and Ca2+ signaling. Annexin A11 dysregulation is involved in the progression, drug-resistance, recurrence of systemic autoimmune disease and cancer.

Publications

Species	Application	Title
mouse	WB	Mol Cell Proteomics; FMRP long-range transport and degradation are mediated by Dynlrb1 in sensory neurons; Authors - Sara Emad El-Agamy
human	WB,IHC	Acta Neuropathol; Annexin A11 aggregation in FTLD-TDP type C and related neurodegenerative disease proteinopathies; Authors - John L Robinson