CoraLite® Plus 488-conjugated Alpha Tubulin Polyclonal antibody

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  IF Staining of HeLa using CL488-14555

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 Alpha Tubulin antibody (CL488-14555) at dilution of 1:200.

Alpha Tubulin Polyclonal Antibody for IF/ICC

Cat No. CL488-14555

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Synonyms

alpha tubulin, Alpha tubulin 3, B ALPHA 1, FLJ25113, LIS3, TUBA1A, TUBA3, Tubulin alpha 1A chain, Tubulin alpha 3 chain, Tubulin B alpha 1, tubulin, alpha 1a

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Tested Applications

• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-14555 targets Alpha Tubulin in IF/ICC applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag6038

  Product name: Recombinant human tubulin-Alpha protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-451 aa of BC050637

  Sequence: MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDSFNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDYEEVGVDSVEGEGEEEGEEY

• Full Name: tubulin, alpha 1a
• Calculated Molecular Weight: 50 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: BC050637
• Gene Symbol: Alpha Tubulin
• Gene ID (NCBI): 7846
• RRID: AB_3672592
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q71U36
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.