Alpha Tubulin Polyclonal antibody

• Validation Data Gallery
• Proteintech vs. Other Vendors

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  WB analysis using 14555-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14555-1-AP (Alpha Tubulin antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse cerebellum using 14555-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 14555-1-AP (alpha Tubulin antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 14555-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14555-1-AP (Alpha Tubulin antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 14555-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Alpha Tubulin antibody (14555-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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  IF Staining of HeLa using 14555-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Alpha Tubulin antibody (14555-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Alpha Tubulin Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 14555-1-AP

Publications(31)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

TUBA1A, Tubulin alpha 1A chain, Alpha-tubulin 3, Detyrosinated tubulin alpha-1A chain, EC:3.6.5.-

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Tested Applications

• Positive WB detected in: HEK-293 cells, mouse cerebellum tissue, HeLa cells, HepG2 cells, NIH/3T3 cells, mouse brain tissue, rat kidney tissue
• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 30 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

14555-1-AP targets Alpha Tubulin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, canine, bovine, sheep
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag6038

  Product name: Recombinant human tubulin-Alpha protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-451 aa of BC050637

  Sequence: MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDSFNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDYEEVGVDSVEGEGEEEGEEY

• Full Name: tubulin, alpha 1a
• Calculated Molecular Weight: 50 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: BC050637
• Gene Symbol: Alpha Tubulin
• Gene ID (NCBI): 7846
• RRID: AB_2212258
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q71U36
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
bovine	WB	Int J Biol Macromol; NR1D1 activation alleviates inflammatory response through inhibition of IL-6 expression in bovine endometrial epithelial cells; Authors - Wanghao Yang
rat	WB	ACS Appl Mater Interfaces; Cerium-Organic Framework and Resveratrol Composite Hydrogel Scaffold with Dual Antioxidant Activity for Enhanced Bone Regeneration; Authors - Yao Lu
human	WB	mBio; Regulation of the WNT-CTNNB1 signaling pathway by severe fever with thrombocytopenia syndrome virus in a cap-snatching manner; Authors - Xia-Ming Jiang
mouse	WB	CNS Neurosci Ther; Plasma-Derived Exosomal i-tRF-LeuCAA as Biomarker for Glioma Diagnosis and Promoter of Epithelial-Mesenchymal Transition via TPM4 Regulation; Authors - Hongyu Liu
mouse	WB	J Neurosci Res; Tau nuclear translocation is a leading step in tau pathology process through P53 stabilization and nucleolar dispersion.; Authors - Shaqayeq Roqanian
human	WB	J Cell Sci; ARF6 protects sister chromatid cohesion to ensure the formation of stable kinetochore-microtubule attachments.; Authors - Mohamed Bourmoum

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Yuting (Verified Customer) (08-27-2025)

Good product

• Applications: Western Blot
• Cell Tissue Type: human