Alpha Tubulin Polyclonal antibody

Alpha Tubulin Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

17

Cat no : 14555-1-AP

Synonyms

acetylated alpha tubulin, TUBA1A, Tubulin alpha 1A chain, EC:3.6.5.-, Detyrosinated tubulin alpha-1A chain

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• Validation Data Gallery
• Proteintech vs. Other Vendors

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  WB analysis of mouse brain using 14555-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14555-1-AP (alpha Tubulin antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse cerebellum using 14555-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 14555-1-AP (alpha Tubulin antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14555-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14555-1-AP (alpha Tubulin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 14555-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14555-1-AP (Alpha Tubulin antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 14555-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Alpha Tubulin antibody (14555-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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  IF Staining of HeLa using 14555-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Alpha Tubulin antibody (14555-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: mouse brain tissue, mouse cerebellum tissue
• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 16 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

14555-1-AP targets Alpha Tubulin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Alpha Tubulin fusion protein Ag6038
• Full Name: tubulin, alpha 1a
• Calculated Molecular Weight: 50 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: BC050637
• Gene Symbol: Alpha Tubulin
• Gene ID (NCBI): 7846
• RRID: AB_2212258
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
bovine	WB	Int J Biol Macromol; NR1D1 activation alleviates inflammatory response through inhibition of IL-6 expression in bovine endometrial epithelial cells; Authors - Wanghao Yang
human	WB	mBio; Regulation of the WNT-CTNNB1 signaling pathway by severe fever with thrombocytopenia syndrome virus in a cap-snatching manner; Authors - Xia-Ming Jiang
mouse	WB	J Neurosci Res; Tau nuclear translocation is a leading step in tau pathology process through P53 stabilization and nucleolar dispersion.; Authors - Shaqayeq Roqanian
human	WB	J Cell Sci; ARF6 protects sister chromatid cohesion to ensure the formation of stable kinetochore-microtubule attachments.; Authors - Mohamed Bourmoum
mouse	WB	Biochim Biophys Acta Mol Basis Dis; Dicer1 promotes Aβ clearance via blocking B2 RNA-mediated repression of apolipoprotein E.; Authors - Yan Wang
human	WB,IF	Exp Cell Res; Identification and characterization of INMAP, a novel interphase nucleus and mitotic apparatus protein that is involved in spindle formation and cell cycle progression.; Authors - Shen Enzhi E