CoraLite® Plus 488-conjugated Alpha Actinin Polyclonal antibody

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• KD/KO Validated

Alpha Actinin Polyclonal Antibody for IF-P, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF-P, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-11313

Synonyms

Alpha actinin 1, ACTN1, Alpha-actinin cytoskeletal isoform, ACTN 1, actinin, alpha 1

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Validation Data Gallery

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  IF Staining of rat heart using CL488-11313

  Immunofluorescent analysis of (4% PFA) fixed rat heart tissue using CoraLite® Plus 488 Alpha Actinin antibody (CL488-11313) at dilution of 1:200.

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  IF Staining of rat heart using CL488-11313

  Immunofluorescent analysis of (4% PFA) fixed rat heart tissue using CoraLite® Plus 488 Alpha Actinin antibody (CL488-11313) at dilution of 1:200.

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  FC experiment of A431 using CL488-11313

  1X10^6 A431 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human Alpha Actinin (CL488-11313) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF-P detected in: rat heart tissue
• Positive FC (Intra) detected in: A431 cells

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-11313 targets Alpha Actinin in IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Alpha Actinin fusion protein Ag1859
• Full Name: actinin, alpha 1
• Calculated Molecular Weight: 103 kDa
• Observed Molecular Weight: 100-105 kDa
• GenBank Accession Number: BC015766
• Gene Symbol: Alpha Actinin
• Gene ID (NCBI): 87
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Alpha Actinins are highly conserved actin-binding proteins that form antiparallel homodimers with the actin-binding domains on each end of the molecule, allowing crosslinking of actin molecules. There are four alpha actinin isoforms identified: two ubiquitously expressed cytoskeletal isoforms (ACTN1 and ACTN4) and two striated muscle-specific sarcomeric isoforms (ACTN2 and ACTN3). ACTN1 is also alternatively spliced to form smooth muscle or brain specific isoforms. This antibody recognize endogenous ACTN1, while it may cross-react with ACTN2 and ACTN4.