Validation Data Gallery
|Positive WB detected in||NIH/3T3 cells, mouse spleen tissue, rat heart tissue, C6 cells|
|Positive IHC detected in||human heart tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||mouse heart tissue, rat heart tissue, C2C12 cells|
|Western Blot (WB)||WB : 1:2000-1:10000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
11313-2-AP targets Alpha Actinin in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Alpha Actinin fusion protein Ag1859|
|Full Name||actinin, alpha 1|
|Calculated molecular weight||103 kDa|
|Observed molecular weight||100-105 kDa|
|GenBank accession number||BC015766|
|Gene ID (NCBI)||87|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Alpha Actinins are highly conserved actin-binding proteins that form antiparallel homodimers with the actin-binding domains on each end of the molecule, allowing crosslinking of actin molecules. There are four alpha actinin isoforms identified: two ubiquitously expressed cytoskeletal isoforms (ACTN1 and ACTN4) and two striated muscle-specific sarcomeric isoforms (ACTN2 and ACTN3). ACTN1 is also alternatively spliced to form smooth muscle or brain specific isoforms. This antibody recognize endogenous ACTN1, while it may cross-react with ACTN2 and ACTN4.
Different roles of BAG3 in cardiac physiological hypertrophy and pathological remodeling.
Aging (Albany NY)
Aging-dependent loss of GAP junction proteins Cx46 and Cx50 in the fiber cells of human and mouse lenses accounts for the diminished coupling conductance.
Adv Sci (Weinh)
MYPT1/PP1-Mediated EZH2 Dephosphorylation at S21 Promotes Epithelial-Mesenchymal Transition in Fibrosis through Control of Multiple Families of Genes.
Proteomic analysis for anti-atherosclerotic effect of tetrahydroxystilbene glucoside in rats.
Biochem Biophys Res Commun
Sirtuin 6 ameliorates alcohol-induced liver injury by reducing endoplasmic reticulum stress in mice.
Biochem Biophys Res Commun
Adipocyte-specific deletion of Depdc5 exacerbates insulin resistance and adipose tissue inflammation in mice.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Jason (Verified Customer) (10-28-2019)
Excellent antibody with consistent results at a 1/100 dilution on formalin fixed tissue.
Steeve (Verified Customer) (01-08-2018)
Tested with another actinin mouse. Gave 1:1 ratio actinin:actinin