Alpha Actinin Polyclonal antibody

Alpha Actinin Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

12

Reviews (2)

Cat no : 11313-2-AP

Synonyms

actinin, alpha 1, ACTN1, alpha Actinin, Alpha actinin 1, F actin cross linking protein, Non muscle alpha actinin 1

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Validation Data Gallery

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  WB analysis using 11313-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11313-2-AP (Alpha Actinin antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat heart using 11313-2-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 11313-2-AP (alpha Actinin antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 11313-2-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 11313-2-AP (alpha Actinin antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 11313-2-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 11313-2-AP (alpha Actinin antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of human heart using 11313-2-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 11313-2-AP (alpha Actinin antibody at dilution of 1:200 (under 10x lens).

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  IF Staining of mouse heart using 11313-2-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 11313-2-AP (alpha Actinin antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). The section was co-stained with 66219-1-Ig (N-cadherin) in red.

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  IF Staining of C2C12 using 11313-2-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed C2C12 cell using 11313-2-AP (alpha Actinin antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of rat heart using 11313-2-AP

  Immunofluorescent analysis of (4% PFA) fixed rat heart tissue using Alpha Actinin antibody (11313-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: NIH/3T3 cells, mouse spleen tissue, rat heart tissue, C6 cells
• Positive IHC detected in: human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: mouse heart tissue, rat heart tissue, C2C12 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 8 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Product Information

11313-2-AP targets Alpha Actinin in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Alpha Actinin fusion protein Ag1859
• Full Name: actinin, alpha 1
• Calculated molecular weight: 103 kDa
• Observed molecular weight: 100-105 kDa
• GenBank accession number: BC015766
• Gene symbol: ACTN1
• Gene ID (NCBI): 87
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Alpha Actinins are highly conserved actin-binding proteins that form antiparallel homodimers with the actin-binding domains on each end of the molecule, allowing crosslinking of actin molecules. There are four alpha actinin isoforms identified: two ubiquitously expressed cytoskeletal isoforms (ACTN1 and ACTN4) and two striated muscle-specific sarcomeric isoforms (ACTN2 and ACTN3). ACTN1 is also alternatively spliced to form smooth muscle or brain specific isoforms. This antibody recognize endogenous ACTN1, while it may cross-react with ACTN2 and ACTN4.

Publications

Species	Application	Title
mouse	IF	Transl Res; Different roles of BAG3 in cardiac physiological hypertrophy and pathological remodeling.; Authors - Pengyu Jia
mouse	WB	Aging (Albany NY); Aging-dependent loss of GAP junction proteins Cx46 and Cx50 in the fiber cells of human and mouse lenses accounts for the diminished coupling conductance.; Authors - Xiao-Dong Gong
human	WB	Adv Sci (Weinh); MYPT1/PP1-Mediated EZH2 Dephosphorylation at S21 Promotes Epithelial-Mesenchymal Transition in Fibrosis through Control of Multiple Families of Genes.; Authors - Lan Zhang
rat	WB	Biomed Pharmacother; Proteomic analysis for anti-atherosclerotic effect of tetrahydroxystilbene glucoside in rats.; Authors - Yao Wenjuan W
mouse	WB	Biochem Biophys Res Commun; Sirtuin 6 ameliorates alcohol-induced liver injury by reducing endoplasmic reticulum stress in mice.; Authors - Yue Xin
mouse	WB	Biochem Biophys Res Commun; Adipocyte-specific deletion of Depdc5 exacerbates insulin resistance and adipose tissue inflammation in mice.; Authors - Jinghong Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Jason (Verified Customer) (10-28-2019)

Excellent antibody with consistent results at a 1/100 dilution on formalin fixed tissue.

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 1/100
• Cell Tissue Type: Mouse myocardium

Alpha Actinin Antibody Immunohistochemistry, validation (1/100 dilution) in Mouse myocardium (Cat no:11313-2-AP)

FH

Steeve (Verified Customer) (01-08-2018)

Tested with another actinin mouse. Gave 1:1 ratio actinin:actinin

• Applications: Western Blot
• Primary Antibody Dilution: 1:1'1000
• Cell Tissue Type: human umbilical veins