Alpha Actinin Polyclonal antibody
Alpha Actinin Polyclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 11313-2-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | NIH/3T3 cells, mouse spleen tissue, rat heart tissue, C6 cells |
Positive IHC detected in | human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | mouse heart tissue, rat heart tissue, C2C12 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 8 publications below |
IHC | See 1 publications below |
IF | See 3 publications below |
Product Information
11313-2-AP targets Alpha Actinin in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Alpha Actinin fusion protein Ag1859 |
Full Name | actinin, alpha 1 |
Calculated molecular weight | 103 kDa |
Observed molecular weight | 100-105 kDa |
GenBank accession number | BC015766 |
Gene symbol | ACTN1 |
Gene ID (NCBI) | 87 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Alpha Actinins are highly conserved actin-binding proteins that form antiparallel homodimers with the actin-binding domains on each end of the molecule, allowing crosslinking of actin molecules. There are four alpha actinin isoforms identified: two ubiquitously expressed cytoskeletal isoforms (ACTN1 and ACTN4) and two striated muscle-specific sarcomeric isoforms (ACTN2 and ACTN3). ACTN1 is also alternatively spliced to form smooth muscle or brain specific isoforms. This antibody recognize endogenous ACTN1, while it may cross-react with ACTN2 and ACTN4.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for Alpha Actinin antibody 11313-2-AP | Download protocol |
IHC protocol for Alpha Actinin antibody 11313-2-AP | Download protocol |
IF protocol for Alpha Actinin antibody 11313-2-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Transl Res Different roles of BAG3 in cardiac physiological hypertrophy and pathological remodeling. | ||
Aging (Albany NY) Aging-dependent loss of GAP junction proteins Cx46 and Cx50 in the fiber cells of human and mouse lenses accounts for the diminished coupling conductance. | ||
Adv Sci (Weinh) MYPT1/PP1-Mediated EZH2 Dephosphorylation at S21 Promotes Epithelial-Mesenchymal Transition in Fibrosis through Control of Multiple Families of Genes. | ||
Biomed Pharmacother Proteomic analysis for anti-atherosclerotic effect of tetrahydroxystilbene glucoside in rats. | ||
Biochem Biophys Res Commun Sirtuin 6 ameliorates alcohol-induced liver injury by reducing endoplasmic reticulum stress in mice. | ||
Biochem Biophys Res Commun Adipocyte-specific deletion of Depdc5 exacerbates insulin resistance and adipose tissue inflammation in mice. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Jason (Verified Customer) (10-28-2019) | Excellent antibody with consistent results at a 1/100 dilution on formalin fixed tissue.
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FH Steeve (Verified Customer) (01-08-2018) | Tested with another actinin mouse. Gave 1:1 ratio actinin:actinin
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