CoraLite® Plus 488-conjugated Alpha Actin Polyclonal antibody

Alpha Actin Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-23660

Synonyms

ACTA, ACTA1, ACTA1 skeletal Muscle Actin, Actin, alpha skeletal muscle, Alpha actin 1, ASMA, CFTD, CFTD1, CFTDM, MPFD, NEM1, NEM2, NEM3

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Validation Data Gallery

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  IF Staining of H9C2 using CL488-23660

  Immunofluorescent analysis of (-20°C Methanol) fixed H9C2 cells using CoraLite® Plus 488 Alpha Actin antibody (CL488-23660) at dilution of 1:200.

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  IF Staining of C2C12 using CL488-23660

  Immunofluorescent analysis of (-20°C Methanol) fixed C2C12 cells using CoraLite® Plus 488 Alpha Actin antibody (CL488-23660) at dilution of 1:200.

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Tested Applications

• Positive IF detected in: H9C2 cells, C2C12 cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-23660 targets Alpha Actin in IF applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Alpha Actin fusion protein Ag19151
• Full Name: actin, alpha 1, skeletal muscle
• Calculated Molecular Weight: 42 kDa
• Observed Molecular Weight: 40-42 kDa
• GenBank Accession Number: BC012597
• Gene Symbol: ACTA1
• Gene ID (NCBI): 58
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.