GST Tag Monoclonal antibody
GST Tag Monoclonal Antibody for WB, IP, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
Recombinant protein, Schistosoma japonicum and More (5)
Applications
WB, IF, IP, CoIP, ELISA
Conjugate
Unconjugated
123
CloneNo.
3G12B10
Cat no : 66001-2-Ig
Synonyms
"GST Tag Antibodies" Comparison
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Tested Applications
| Positive WB detected in | Recombinant protein |
| Positive IP detected in | E. coli lysate |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 103 publications below |
| IF | See 4 publications below |
| IP | See 24 publications below |
| CoIP | See 5 publications below |
Product Information
66001-2-Ig targets GST Tag in WB, IF, IP, CoIP, ELISA applications and shows reactivity with Recombinant protein, Schistosoma japonicum samples.
| Tested Reactivity | Recombinant protein, Schistosoma japonicum |
| Cited Reactivity | human, mouse, rat, monkey, chicken |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Recombinant protein |
| Full Name | GST Tag |
| Calculated Molecular Weight | 26 kDa |
| GenBank Accession Number | FN315687 |
| Gene Symbol | |
| Gene ID (NCBI) | |
| RRID | AB_2881488 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. GST (Glutathione S-Transferase) is a widely used protein tag encoded by the Schistosoma japonicum. GST provides both an easily detectable tag and a simple purification process with little effect on the biological function of the protein of interest. Antibodies to GST are useful for checking protein expression both in plaques and on Western blots as well as for immunoaffinity purification of proteins expressed in cells. This antibody recognizes the GST-tag fused to either the N- or C-terminus of targeted proteins.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GST Tag antibody 66001-2-Ig | Download protocol |
| IP protocol for GST Tag antibody 66001-2-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Food Identification and knockout of a herbivore susceptibility gene enhances planthopper resistance and increases rice yield | ||
Nat Cancer METTL16 antagonizes MRE11-mediated DNA end resection and confers synthetic lethality to PARP inhibition in pancreatic ductal adenocarcinoma | ||
Nat Cell Biol Phagolysosome resolution requires contacts with the endoplasmic reticulum and phosphatidylinositol-4-phosphate signalling. | ||
Sci Bull (Beijing) Targeted clearance of mitochondria by an autophagy-tethering compound (ATTEC) and its potential therapeutic effects | ||
Nat Commun Fungal secondary metabolism is governed by an RNA-binding protein CsdA/RsdA complex | ||
Nat Commun N-terminal α-amino SUMOylation of cofilin-1 is critical for its regulation of actin depolymerization |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Lana (Verified Customer) (05-27-2022) | SDS-PAGE: 4-12% Bis-Tris gradient gel. Transfer: Immobilon-FL transfer membranes (Millipore) O/N at 30V, 4C. Blocking: SEA Block Blocking Buffer 1h, room T. Primary Ab: O/N incubation at 4C, 1:1000. Secondary Ab: IRDye 680LT Goat anti-Mouse, 1:15000. Lines of WB image: 1 – protein ladder, 2 and 3 – GST protein different amounts.
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FH Susanne (Verified Customer) (11-18-2021) | Clear Signal, no background on E.coli lysate with expressed GST blotted on PVDF membrane
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FH Aurelie (Verified Customer) (12-11-2018) |
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