Acetyl-Histone H3 (Lys23) Recombinant antibody, PBS Only

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  IHC staining of mouse testis using 82838-2-RR (same clone as 82838-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 82838-2-RR (HIST1H3A antibody) at dilution of 1:400 (under 10x lens). This data was developed using the same antibody clone with 82838-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 82838-2-RR (same clone as 82838-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 82838-2-RR (Acetyl-Histone H3 (Lys23) antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82838-2-PBS in a different storage buffer formulation.

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  IHC staining of rat testis using 82838-2-RR (same clone as 82838-2-PBS)

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 82838-2-RR (Acetyl-Histone H3 (Lys23) antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82838-2-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 82838-2-RR (same clone as 82838-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Acetyl-Histone H3 (Lys23) antibody (82838-2-RR, Clone: 241137E4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82838-2-PBS in a different storage buffer formulation.

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  Dot Blot experiment of peptide using 82838-2-RR (same clone as 82838-2-PBS)

  Dot blot analysis was used to confirm the specificity of Acetyl-Histone H3 (Lys23) antibody. Acetylated peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row. This data was developed using the same antibody clone with 82838-2-PBS in a different storage buffer formulation.

Acetyl-Histone H3 (Lys23) Uni-rAb^TM Recombinant Antibody for IHC, IF/ICC, Dot Blot, Indirect ELISA

Cat No. 82838-2-PBS

Clone No.241137E4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF/ICC, Dot Blot, Indirect ELISA

Synonyms

HIST1H3A, 241137E4, Histone-H3, HistoneH3, Histone H3

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Product Information

82838-2-PBS targets Acetyl-Histone H3 (Lys23) in IHC, IF/ICC, Dot Blot, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: histone cluster 1, H3a
• GenBank Accession Number: BC066245
• Gene Symbol: HIST1H3A
• Gene ID (NCBI): 8350
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P68431
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.