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Acetyl-Histone H3 (Lys23) Recombinant antibody

Acetyl-Histone H3 (Lys23) Uni-rAbTM Recombinant Antibody for IHC, IF/ICC, Dot Blot, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, Dot Blot, ELISA

Conjugate

Unconjugated

CloneNo.

241137E4

Cat no : 82838-2-RR

Synonyms

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse testis tissue using Acetyl-Histone H3 (Lys23) Recombinant antibody (82838-2-RR)

    IHC staining of mouse testis using 82838-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 82838-2-RR (HIST1H3A antibody) at dilution of 1:400 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Acetyl-Histone H3 (Lys23) Recombinant antibody (82838-2-RR)

    IF Staining of HeLa using 82838-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Acetyl-Histone H3 (Lys23) antibody (82838-2-RR, Clone: 241137E4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Dot Blot experiment of peptide using Acetyl-Histone H3 (Lys23) Recombinant antibody (82838-2-RR)

    Dot Blot experiment of peptide using 82838-2-RR

    Dot blot analysis was used to confirm the specificity of Acetyl-Histone H3 (Lys23) antibody. Acetylated peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row.

Tested Applications

Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive Dot Blot detected inpeptide

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
DOT BLOTDOT BLOT : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82838-2-RR targets Acetyl-Histone H3 (Lys23) in IHC, IF/ICC, Dot Blot, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide
Full Name histone cluster 1, H3a
GenBank Accession NumberBC066245
Gene Symbol HIST1H3A
Gene ID (NCBI) 8350
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for Acetyl-Histone H3 (Lys23) antibody 82838-2-RRDownload protocol
IF protocol for Acetyl-Histone H3 (Lys23) antibody 82838-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols