Acetyl-Histone H3 (Lys23) Recombinant antibody

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  IHC staining of mouse testis using 82838-2-RR

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 82838-2-RR (HIST1H3A antibody) at dilution of 1:400 (under 10x lens).

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  IHC staining of mouse testis using 82838-2-RR

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 82838-2-RR (Acetyl-Histone H3 (Lys23) antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat testis using 82838-2-RR

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 82838-2-RR (Acetyl-Histone H3 (Lys23) antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 82838-2-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Acetyl-Histone H3 (Lys23) antibody (82838-2-RR, Clone: 241137E4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Dot Blot experiment of peptide using 82838-2-RR

  Dot blot analysis was used to confirm the specificity of Acetyl-Histone H3 (Lys23) antibody. Acetylated peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row.

Acetyl-Histone H3 (Lys23) Recombinant Antibody for IHC, IF/ICC, Dot Blot, ELISA

Cat No. 82838-2-RR

Clone No.241137E4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF/ICC, Dot Blot, ELISA

Synonyms

HIST1H3A, 241137E4, Histone-H3, HistoneH3, Histone H3

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Tested Applications

• Positive IHC detected in: mouse testis tissue, rat testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells
• Positive Dot Blot detected in: peptide

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• DOT BLOT: DOT BLOT : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82838-2-RR targets Acetyl-Histone H3 (Lys23) in IHC, IF/ICC, Dot Blot, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: histone cluster 1, H3a
• GenBank Accession Number: BC066245
• Gene Symbol: HIST1H3A
• Gene ID (NCBI): 8350
• RRID: AB_3670572
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P68431
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.