Acetyl-Histone H2B (Lys16) Recombinant antibody

Acetyl-Histone H2B (Lys16) Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, Dot Blot, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, Dot Blot, ELISA

Conjugate

Unconjugated

CloneNo.

241194H2

Cat no : 84446-1-RR

Synonyms

HIST2H2BE, Histone H2B, H2BC21, H2B/q, H2B.1

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Validation Data Gallery

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  WB analysis of NIH/3T3 using 84446-1-RR

  Trichostatin A treated and untreated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 84446-1-RR (Acetyl-Histone H2B (Lys16) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control.

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  IF Staining of HeLa using 84446-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Histone H2B antibody (84446-1-RR, Clone: 241194H2 ) at dilution of 1:850 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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  Dot Blot experiment of peptide using 84446-1-RR

  Dot blot analysis was used to confirm the specificity of Acetyl-Histone H2B (Lys16) antibody. Acetylated peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row.

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Tested Applications

• Positive WB detected in: Trichostatin A treated NIH/3T3 cells
• Positive IF/ICC detected in: HeLa cells
• Positive Dot Blot detected in: peptide

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:425-1:1700
• DOT BLOT: DOT BLOT : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84446-1-RR targets Acetyl-Histone H2B (Lys16) in WB, IF/ICC, Dot Blot, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: histone cluster 2, H2be
• Calculated Molecular Weight: 14 kDa
• Observed Molecular Weight: 15 kDa
• GenBank Accession Number: BC005827
• Gene Symbol: Histone H2B
• Gene ID (NCBI): 8349
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.