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Acetyl-Histone H2B (Lys16) Recombinant antibody, PBS Only

Acetyl-Histone H2B (Lys16) Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Dot Blot, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, Dot Blot, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241194H2

Cat no : 84446-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of NIH/3T3 cells using Acetyl-Histone H2B (Lys16) Recombinant antibody (84446-1-RR)

    WB analysis of NIH/3T3 using 84446-1-RR (same clone as 84446-1-PBS)

    Trichostatin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 84446-1-RR (Histone H2B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84446-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Acetyl-Histone H2B (Lys16) Recombinant antibody (84446-1-RR)

    IF Staining of HeLa using 84446-1-RR (same clone as 84446-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Histone H2B antibody (84446-1-RR, Clone: 241194H2 ) at dilution of 1:850 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 84446-1-PBS in a different storage buffer formulation.

  • Dot Blot experiment of peptide using Acetyl-Histone H2B (Lys16) Recombinant antibody (84446-1-RR)

    Dot Blot experiment of peptide using 84446-1-RR (same clone as 84446-1-PBS)

    Dot blot analysis was used to confirm the specificity of Acetyl-Histone H2B (Lys16) antibody. Acetylated peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row. This data was developed using the same antibody clone with 84446-1-PBS in a different storage buffer formulation.

Product Information

84446-1-PBS targets Acetyl-Histone H2B (Lys16) in WB, IF/ICC, Dot Blot, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide
Full Name histone cluster 2, H2be
Calculated Molecular Weight 14 kDa
Observed Molecular Weight15 kDa
GenBank Accession NumberBC005827
Gene Symbol Histone H2B
Gene ID (NCBI) 8349
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.