ATP1B1 Recombinant antibody, PBS Only

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  WB analysis using 84707-5-RR (same clone as 84707-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84707-5-RR (ATP1B1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84707-5-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 84707-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84707-5-RR against Human ATP1B1 were performed. The affinity constant is below 1 pM.

ATP1B1 Uni-rAb^TM Recombinant Antibody for WB, Indirect ELISA

Cat No. 84707-5-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

Sodium/potassium-dependent ATPase subunit beta-1, Na+/K+ ATPase beta 1 subunit, Na, K-ATPase β1, Beta 1 subunit of Na(+) K(+) ATPase, ATP1B 1

Formulation:  PBS Only

PBS and Azide

PBS Only

Conjugate:  Unconjugated

Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

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Product Information

84707-5-PBS targets ATP1B1 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ATP1B1 fusion protein Ag7279
• Full Name: ATPase, Na+/K+ transporting, beta 1 polypeptide
• Calculated Molecular Weight: 35 kDa
• Observed Molecular Weight: 45-52 kDa
• GenBank Accession Number: BC000006
• Gene Symbol: ATP1B1
• Gene ID (NCBI): 481
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P05026
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

ATP1B1 is one of beta subunits of the Na+/K+ ATPase and responsible for formation and structural integrity of the Na+/K+ ATPase. The Na+/K+ ATPase is a plasma membrane pump consisting of alpha, beta, and gamma subunits. At least four of Na+/K+-ATPase beta subunits (β1, β2, β3, β4) have been identified in mammalian cells; the β1-subunit (ATP1B1) is the most ubiquitous. The Na+/K+ ATPase β subunits have multiple N-glycosylation sites. The predicted MW of ATP1B1 is 35 kDa, while it migrates around 40-52 kDa due to the variable glycosylation. (PMID: 10896885, 17714085)