Biotin Plus-conjugated ATP1A2-Specific Polyclonal antibody

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  IHC staining of mouse heart using Biotin-55179

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using Biotin-55179 (ATP1A2-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

ATP1A2-Specific Polyclonal Antibody for IHC

Cat No. Biotin-55179

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC

Synonyms

ATP1A2, Sodium pump subunit alpha-2, Na(+)/K(+) ATPase alpha-2 subunit, EC:7.2.2.13, ATP1A 2

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Tested Applications

• Positive IHC detected in: mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

Biotin-55179 targets ATP1A2-Specific in IHC applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: ATPase, Na+/K+ transporting, alpha 2 (+) polypeptide
• Calculated Molecular Weight: 112 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: NM_000702
• Gene Symbol: ATP1A2
• Gene ID (NCBI): 477
• Conjugate: Biotin Plus
• Form: Liquid
• Purification Method: Antigen Affinity Purified
• UNIPROT ID: P50993
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ATP1A2, also named as KIAA0778, belongs to the cation transport ATPase (P-type) family and Type IIC subfamily. It is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. Defects in ATP1A2 are the cause of familial hemiplegic migraine type 2 (FHM2). Defects in ATP1A2 are a cause of alternating hemiplegia of childhood (AHC). This antibody is specific to ATP1A2.