ATP1A2-Specific Polyclonal antibody

• Featured Product
• KD/KO Validated

ATP1A2-Specific Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 55179-1-AP

Synonyms

ATP1A2, ATP1A2-Specific, FHM2, KIAA0778, MHP2, Sodium pump subunit alpha 2

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Validation Data Gallery

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  WB analysis of mouse brain using 55179-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55179-1-AP (ATP1A2-Specific antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 55179-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 55179-1-AP (ATP1A2-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 55179-1-AP

  IP result of anti-ATP1A2-Specific (IP:55179-1-AP, 4ug; Detection:55179-1-AP 1:800) with mouse brain tissue lysate 4000ug.

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  IHC staining of human skeletal muscle using 55179-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 55179-1-AP (ATP1A2-Specific antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skeletal muscle using 55179-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 55179-1-AP (ATP1A2-Specific antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse heart using 55179-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart using 55179-1-AP (ATP1A2-Specific antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of mouse heart using 55179-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart using 55179-1-AP (ATP1A2-Specific antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse brain tissue, human brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human skeletal muscle tissue, mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 1 publications below

Product Information

55179-1-AP targets ATP1A2-Specific in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: ATPase, Na+/K+ transporting, alpha 2 (+) polypeptide
• Calculated Molecular Weight: 112 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: NM_000702
• Gene Symbol: ATP1A2
• Gene ID (NCBI): 477
• RRID: AB_2881284
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATP1A2, also named as KIAA0778, belongs to the cation transport ATPase (P-type) family and Type IIC subfamily. It is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. Defects in ATP1A2 are the cause of familial hemiplegic migraine type 2 (FHM2). Defects in ATP1A2 are a cause of alternating hemiplegia of childhood (AHC). This antibody is specific to ATP1A2.

Publications

Species	Application	Title
mouse	WB,IF	Cell Calcium; α2 isoform of Na+,K+-ATPase via Na+,Ca2+ exchanger modulates myelin basic protein synthesis in oligodendrocyte lineage cells in vitro.; Authors - Jens Hammann ; KD Validated
rat	WB	Cell Calcium; Na+/Ca2+ exchanger isoform 1 (NCX1) and canonical transient receptor potential channel 6 (TRPC6) are recruited by STIM1 to mediate Store-Operated Calcium Entry in primary cortical neurons.; Authors - Valentina Tedeschi