CoraLite® Plus 488-conjugated ATG9A Monoclonal antibody

ATG9A Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1D12E11

Cat no : CL488-67096

Synonyms

APG9 like 1, APG9L1, ATG9A, Autophagy related protein 9A, MGD3208

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Validation Data Gallery

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  IF Staining of HeLa using CL488-67096

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488-conjugated ATG9A antibody (CL488-67096, Clone: 1D12E11 ) at dilution of 1:200, CL594-Phalloidin (red).

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  IF Staining of HepG2 using CL488-67096

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CL488-67096 (ATG9A antibody) at dilution of 1:100.

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Tested Applications

• Positive IF/ICC detected in: HeLa cells, HepG2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67096 targets ATG9A in IF/ICC applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ATG9A fusion protein Ag24278
• Full Name: ATG9 autophagy related 9 homolog A (S. cerevisiae)
• Calculated Molecular Weight: 837 aa, 94 kDa
• Observed Molecular Weight: 94 kDa
• GenBank Accession Number: BC065534
• Gene Symbol: ATG9A
• Gene ID (NCBI): 79065
• RRID: AB_2883397
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ATG9A is the only transmembrane ATG protein essential for autophagy. It plays a key role in the organization of the preautophagosomal structure/phagophore assembly site (PAS). It has been reported that ATG9A expression is increased in oral squamous cell carcinoma and breast cancers. The inhibition of ATG9A can lead to an inhibition of cancer cell proliferation and invasion.(PMID: 29437695, 29568063)