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ATF6 Recombinant antibody, PBS Only

ATF6 Recombinant Antibody for WB, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2O8

Cat no : 82278-1-PBS

Synonyms

ATF6, ATF6 alpha, ATF6A

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using ATF6 Recombinant antibody (82278-1-RR)

    WB analysis of HeLa using 82278-1-RR (same clone as 82278-1-PBS)

    WB result of ATF6 antibody (82278-1-RR; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATF6 transfected HeLa cells. This data was developed using the same antibody clone with 82278-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ATF6 Recombinant antibody (82278-1-RR)

    WB analysis using 82278-1-RR (same clone as 82278-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82278-1-RR (ATF6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82278-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using ATF6 Recombinant antibody (82278-1-RR)

    IP experiment of HeLa using 82278-1-RR (same clone as 82278-1-PBS)

    IP result of anti-ATF6 (IP:82278-1-RR, 4ug; Detection:82278-1-RR 1:2000) with HeLa cells lysate 1280 ug. This data was developed using the same antibody clone with 82278-1-PBS in a different storage buffer formulation.

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82278-1-PBS targets ATF6 in WB, IP, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ATF6 fusion protein Ag21456
Full Name activating transcription factor 6
Calculated Molecular Weight 75 kDa
Observed Molecular Weight90-100 kDa
GenBank Accession NumberBC014969
Gene Symbol ATF6
Gene ID (NCBI) 22926
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Activating transcription factor 6 (ATF6) is a transcription factor that acts during endoplasmic reticulum stress by activating unfolded protein response target genes. Binds DNA on the 5'-CCAC[GA]-3'half of the ER stress response element (ERSE) (5'-CCAAT-N(9)-CCAC[GA]-3') and of ERSE II (5'-ATTGG-N-CCACG-3'). Binding to ERSE requires binding of NF-Y to ERSE. Could also be involved in activation of transcription by the serum response factor.During unfolded protein response an approximative 50 kDa fragment containing the cytoplasmic transcription factor domain is released by proteolysis. The cleavage seems to be performed sequentially by site-1 and site-2 proteases. The fully glycosylated form of ATF6, a 670 amino acid protein, exhibits an electrophoretic mobility of ~90 kDa in denaturing SDS-gels, in part because of the glycosylated modifications. ATF6 has 3 consensus sites for N-linked glycosylation and exists constitutively as a glycosylated protein. Differentially glycosylated ATF6 forms may result from mutations or experimental treatment (PMID:15804611) (PMID:14699159).