ATF6 Recombinant antibody

• Featured Product
• KD/KO Validated

ATF6 Uni-rAb^TM Recombinant Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Conjugate

Unconjugated

CloneNo.

2O8

Cat no : 82278-1-RR

Synonyms

ATF6-alpha, ATF6A, ATF6 alpha, ATF 6, activating transcription factor 6

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Validation Data Gallery

• 

  WB analysis using 82278-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82278-1-RR (ATF6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 82278-1-RR

  WB result of ATF6 antibody (82278-1-RR; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATF6 transfected HeLa cells.

• 

  IP experiment of HeLa using 82278-1-RR

  IP result of anti-ATF6 (IP:82278-1-RR, 4ug; Detection:82278-1-RR 1:2000) with HeLa cells lysate 1280 ug.

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  Affinity and Kinetic Characterization of 82278-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82278-1-RR against Human ATF6 were performed. The affinity constant is 0.344 nM.

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, MCF-7 cells, Jurkat cells, K-562 cells
• Positive IP detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82278-1-RR targets ATF6 in WB, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ATF6 fusion protein Ag21456
• Full Name: activating transcription factor 6
• Calculated Molecular Weight: 75 kDa
• Observed Molecular Weight: 90-100 kDa
• GenBank Accession Number: BC014969
• Gene Symbol: ATF6
• Gene ID (NCBI): 22926
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Activating transcription factor 6 (ATF6) is a transcription factor that acts during endoplasmic reticulum stress by activating unfolded protein response target genes. Binds DNA on the 5'-CCAC[GA]-3'half of the ER stress response element (ERSE) (5'-CCAAT-N(9)-CCAC[GA]-3') and of ERSE II (5'-ATTGG-N-CCACG-3'). Binding to ERSE requires binding of NF-Y to ERSE. Could also be involved in activation of transcription by the serum response factor.During unfolded protein response an approximative 50 kDa fragment containing the cytoplasmic transcription factor domain is released by proteolysis. The cleavage seems to be performed sequentially by site-1 and site-2 proteases. The fully glycosylated form of ATF6, a 670 amino acid protein, exhibits an electrophoretic mobility of ~90 kDa in denaturing SDS-gels, in part because of the glycosylated modifications. ATF6 has 3 consensus sites for N-linked glycosylation and exists constitutively as a glycosylated protein. Differentially glycosylated ATF6 forms may result from mutations or experimental treatment (PMID:15804611) (PMID:14699159).