CoraLite® Plus 488-conjugated ASNS Monoclonal antibody

ASNS Monoclonal Antibody for WB, IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1C3D3

Cat no : CL488-68004

Synonyms

TS11, Glutamine-dependent asparagine synthetase, EC:6.3.5.4, Asparagine synthetase [glutamine-hydrolyzing], 1C3D3

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Validation Data Gallery

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  WB analysis using CL488-68004

  Various lysates were subjected to SDS PAGE followed by western blot with CL488-68004 (ASNS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of SH-SY5Y using CL488-68004

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using CoraLite® Plus 488 ASNS antibody (CL488-68004, Clone: 1C3D3 ) at dilution of 1:200.

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Tested Applications

• Positive WB detected in: LNCaP cells, K-562 cells
• Positive IF/ICC detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68004 targets ASNS in WB, IF/ICC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ASNS fusion protein Ag6817
• Full Name: asparagine synthetase
• Calculated Molecular Weight: 64 kDa
• Observed Molecular Weight: 55-64 kDa
• GenBank Accession Number: BC014621
• Gene Symbol: ASNS
• Gene ID (NCBI): 440
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Asparagine synthetase [glutamine-hydrolyzing](ASNS) is also named as TS11. The asparagine synthetase gene, encoding the enzyme that catalyzes the synthesis of asparagine and glutamate using glutamine and aspartate, is a gene for which transcription is highly regulated by the nutritional status of the cell(PMID:15385533). ASNS has some isoforms with the MW of 55-64 kDa.