ASNS Monoclonal antibody

ASNS Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1C3D3

Cat no : 68004-1-Ig

Synonyms

ASNS, asparagine synthetase, TS11



Tested Applications

Positive WB detected inK-562 cells, A431 cells, LNCaP cells, HeLa cells, HEK-293 cells, NIH/3T3 cells, 4T1 cells
Positive IHC detected inrat pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

68004-1-Ig targets ASNS in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ASNS fusion protein Ag6817
Full Name asparagine synthetase
Calculated molecular weight 64 kDa
Observed molecular weight 55-64 kDa
GenBank accession numberBC014621
Gene symbol ASNS
Gene ID (NCBI) 440
RRIDAB_2918751
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Asparagine synthetase [glutamine-hydrolyzing](ASNS) is also named as TS11. The asparagine synthetase gene, encoding the enzyme that catalyzes the synthesis of asparagine and glutamate using glutamine and aspartate, is a gene for which transcription is highly regulated by the nutritional status of the cell(PMID:15385533). ASNS has some isoforms with the MW of 55-64 kDa.

Protocols

Product Specific Protocols
WB protocol for ASNS antibody 68004-1-IgDownload protocol
IHC protocol for ASNS antibody 68004-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols