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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated ASNS Polyclonal antibody

ASNS Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-14681

Synonyms

ASNS, asparagine synthetase, TS11

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using CoraLite® Plus 488-conjugated ASNS Polyclonal anti (CL488-14681)

    IF Staining of SH-SY5Y using CL488-14681

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using CoraLite® Plus 488 ASNS antibody (CL488-14681) at dilution of 1:200.

Tested Applications

Positive IF/ICC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-14681 targets ASNS in IF/ICC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ASNS fusion protein Ag6393
Full Name asparagine synthetase
Calculated Molecular Weight 64 kDa
Observed Molecular Weight 55-64 kDa
GenBank Accession NumberBC014621
Gene Symbol ASNS
Gene ID (NCBI) 440
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Asparagine synthetase [glutamine-hydrolyzing](ASNS) is also named as TS11. The asparagine synthetase gene, encoding the enzyme that catalyzes the synthesis of asparagine and glutamate using glutamine and aspartate, is a gene for which transcription is highly regulated by the nutritional status of the cell(PMID:15385533). ASNS has some isoforms with the MW of 55-64 kDa.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 ASNS antibody CL488-14681Download protocol
Standard Protocols
Click here to view our Standard Protocols