• Featured Product
  • KD/KO Validated

ASNS Polyclonal antibody

ASNS Polyclonal Antibody for IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human and More (2)





Cat no : 14681-1-AP


ASNS, asparagine synthetase, TS11

Tested Applications

Positive IP detected inHeLa cells
Positive IHC detected inhuman retinoblastoma tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inSH-SY5Y cells

Recommended dilution

Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14681-1-AP targets ASNS in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ASNS fusion protein Ag6393
Full Name asparagine synthetase
Calculated Molecular Weight 64 kDa
Observed Molecular Weight 55-64 kDa
GenBank Accession NumberBC014621
Gene Symbol ASNS
Gene ID (NCBI) 440
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Asparagine synthetase [glutamine-hydrolyzing](ASNS) is also named as TS11. The asparagine synthetase gene, encoding the enzyme that catalyzes the synthesis of asparagine and glutamate using glutamine and aspartate, is a gene for which transcription is highly regulated by the nutritional status of the cell(PMID:15385533). ASNS has some isoforms with the MW of 55-64 kDa.


Product Specific Protocols
WB protocol for ASNS antibody 14681-1-APDownload protocol
IHC protocol for ASNS antibody 14681-1-APDownload protocol
IF protocol for ASNS antibody 14681-1-APDownload protocol
IP protocol for ASNS antibody 14681-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Cell Metab

ZBTB1 Regulates Asparagine Synthesis and Leukemia Cell Response to L-Asparaginase.

Authors - Robert T Williams

Cell Metab

Asparagine couples mitochondrial respiration to ATF4 activity and tumor growth.

Authors - Abigail S Krall
  • KO Validated

Mol Cell

Filamentous GLS1 promotes ROS-induced apoptosis upon glutamine deprivation via insufficient asparagine synthesis.

Authors - Bin Jiang
  • KD Validated
humanWB, IHC

Mol Cell

Asparagine plays a critical role in regulating cellular adaptation to glutamine depletion.

Authors - Ji Zhang
  • KD Validated

Nat Commun

p53-mediated control of aspartate-asparagine homeostasis dictates LKB1 activity and modulates cell survival.

Authors - Longfei Deng
  • KD Validated

J Exp Med

Glutamine depletion regulates Slug to promote EMT and metastasis in pancreatic cancer.

Authors - Maria Victoria Recouvreux


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Daniel (Verified Customer) (12-02-2019)

Human RCC4 kidney cancer cell lysate was denatured in SDS-sample buffer and size-separated on a denaturing gel.A specific band appeared for ASNS.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Human kidney cancer cells (RCC4)
ASNS Antibody Western Blot, validation (1:1000 dilution) in Human kidney cancer cells (RCC4) (Cat no:14681-1-AP)

Daniel (Verified Customer) (12-02-2019)

Mouse kidney stained for Asns (green, AF488) and nucleus (blue, DAPI).

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: Mouse kidney
ASNS Antibody Immunofluorescence, validation (1:200 dilution) in Mouse kidney (Cat no:14681-1-AP)