ASNS Polyclonal antibody

• Featured Product
• KD/KO Validated

ASNS Polyclonal Antibody for IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

56

Reviews (2)

Cat no : 14681-1-AP

Synonyms

TS11, Glutamine-dependent asparagine synthetase, EC:6.3.5.4, Asparagine synthetase [glutamine-hydrolyzing]

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Validation Data Gallery

• 

  WB analysis of Jurkat using 14681-1-AP

  WB result of ASNS antibody (14681-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ASNS transfected Jurkat cells.

• 

  WB analysis using 14681-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14681-1-AP (ASNS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 14681-1-AP

  IP result of anti-ASNS (IP:14681-1-AP, 4ug; Detection:14681-1-AP 1:500) with HeLa cells lysate 1200ug.

• 

  IHC staining of human retinoblastoma using 14681-1-AP

  Immunohistochemical analysis of paraffin-embedded human retinoblastoma tissue slide using 14681-1-AP (ASNS Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human retinoblastoma using 14681-1-AP

  Immunohistochemical analysis of paraffin-embedded human retinoblastoma tissue slide using 14681-1-AP (ASNS Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 14681-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14681-1-AP (ASNS Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 14681-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14681-1-AP (ASNS Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of K-562 using 14681-1-AP

  Immunofluorescent analysis of (4% PFA) fixed K-562 cells using ASNS antibody (14681-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Tested Applications

• Positive IP detected in: HeLa cells
• Positive IHC detected in: human retinoblastoma tissue, human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: K-562 cells

Recommended dilution

• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 15 publications below
• WB: See 48 publications below
• IHC: See 10 publications below
• IF: See 2 publications below

Product Information

14681-1-AP targets ASNS in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ASNS fusion protein Ag6393
• Full Name: asparagine synthetase
• Calculated Molecular Weight: 64 kDa
• Observed Molecular Weight: 55-64 kDa
• GenBank Accession Number: BC014621
• Gene Symbol: ASNS
• Gene ID (NCBI): 440
• RRID: AB_2060119
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Asparagine synthetase [glutamine-hydrolyzing](ASNS) is also named as TS11. The asparagine synthetase gene, encoding the enzyme that catalyzes the synthesis of asparagine and glutamate using glutamine and aspartate, is a gene for which transcription is highly regulated by the nutritional status of the cell(PMID:15385533). ASNS has some isoforms with the MW of 55-64 kDa.

Publications

Species	Application	Title
human	WB	Cell Metab; ZBTB1 Regulates Asparagine Synthesis and Leukemia Cell Response to L-Asparaginase.; Authors - Robert T Williams
human	WB	Cell Metab; Asparagine couples mitochondrial respiration to ATF4 activity and tumor growth.; Authors - Abigail S Krall ; KO Validated
human	WB	Mol Cell; Filamentous GLS1 promotes ROS-induced apoptosis upon glutamine deprivation via insufficient asparagine synthesis.; Authors - Bin Jiang ; KD Validated
human	WB, IHC	Mol Cell; Asparagine plays a critical role in regulating cellular adaptation to glutamine depletion.; Authors - Ji Zhang ; KD Validated
human	WB	Nat Commun; p53-mediated control of aspartate-asparagine homeostasis dictates LKB1 activity and modulates cell survival.; Authors - Longfei Deng ; KD Validated
human	IHC	J Exp Med; Glutamine depletion regulates Slug to promote EMT and metastasis in pancreatic cancer.; Authors - Maria Victoria Recouvreux

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Daniel (Verified Customer) (12-02-2019)

Human RCC4 kidney cancer cell lysate was denatured in SDS-sample buffer and size-separated on a denaturing gel.A specific band appeared for ASNS.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human kidney cancer cells (RCC4)

ASNS Antibody Western Blot, validation (1:1000 dilution) in Human kidney cancer cells (RCC4) (Cat no:14681-1-AP)

FH

Daniel (Verified Customer) (12-02-2019)

Mouse kidney stained for Asns (green, AF488) and nucleus (blue, DAPI).

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Mouse kidney

ASNS Antibody Immunofluorescence, validation (1:200 dilution) in Mouse kidney (Cat no:14681-1-AP)

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