ASNS Monoclonal antibody, PBS Only

ASNS Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1C3D3

Cat no : 68004-1-PBS

Synonyms

TS11, Glutamine-dependent asparagine synthetase, EC:6.3.5.4, Asparagine synthetase [glutamine-hydrolyzing], 1C3D3

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Validation Data Gallery

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  WB analysis of K-562 using 68004-1-Ig (same clone as 68004-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 68004-1-Ig (ASNS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68004-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68004-1-Ig (same clone as 68004-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 68004-1-Ig (ASNS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68004-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 68004-1-Ig (same clone as 68004-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 68004-1-Ig (ASNS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68004-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 68004-1-Ig (same clone as 68004-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 68004-1-Ig (ASNS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68004-1-PBS in a different storage buffer formulation.

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  WB analysis of LNCaP using 68004-1-Ig (same clone as 68004-1-PBS)

  LNCaP cells were subjected to SDS PAGE followed by western blot with 68004-1-Ig (ASNS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68004-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A431 using 68004-1-Ig (same clone as 68004-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 68004-1-Ig (ASNS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68004-1-PBS in a different storage buffer formulation.

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  IHC staining of rat pancreas using 68004-1-Ig (same clone as 68004-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 68004-1-Ig (ASNS antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68004-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat pancreas using 68004-1-Ig (same clone as 68004-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 68004-1-Ig (ASNS antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68004-1-PBS in a different storage buffer formulation.

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  IF Staining of SH-SY5Y using 68004-1-Ig (same clone as 68004-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using ASNS antibody (68004-1-Ig, Clone: 1C3D3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68004-1-PBS in a different storage buffer formulation.

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Product Information

68004-1-PBS targets ASNS in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ASNS fusion protein Ag6817
• Full Name: asparagine synthetase
• Calculated Molecular Weight: 64 kDa
• Observed Molecular Weight: 55-64 kDa
• GenBank Accession Number: BC014621
• Gene Symbol: ASNS
• Gene ID (NCBI): 440
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Asparagine synthetase [glutamine-hydrolyzing](ASNS) is also named as TS11. The asparagine synthetase gene, encoding the enzyme that catalyzes the synthesis of asparagine and glutamate using glutamine and aspartate, is a gene for which transcription is highly regulated by the nutritional status of the cell(PMID:15385533). ASNS has some isoforms with the MW of 55-64 kDa.