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ASNS Monoclonal antibody

ASNS Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1C3D3

Cat no : 68004-1-Ig

Synonyms

TS11, Glutamine-dependent asparagine synthetase, EC:6.3.5.4, Asparagine synthetase [glutamine-hydrolyzing], 1C3D3

Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using ASNS Monoclonal antibody (68004-1-Ig)

    WB analysis of K-562 using 68004-1-Ig

    K-562 cells were subjected to SDS PAGE followed by western blot with 68004-1-Ig (ASNS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using ASNS Monoclonal antibody (68004-1-Ig)

    WB analysis using 68004-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 68004-1-Ig (ASNS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using ASNS Monoclonal antibody (68004-1-Ig)

    WB analysis of HEK-293 using 68004-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 68004-1-Ig (ASNS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using ASNS Monoclonal antibody (68004-1-Ig)

    WB analysis of HeLa using 68004-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 68004-1-Ig (ASNS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of LNCaP cells using ASNS Monoclonal antibody (68004-1-Ig)

    WB analysis of LNCaP using 68004-1-Ig

    LNCaP cells were subjected to SDS PAGE followed by western blot with 68004-1-Ig (ASNS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using ASNS Monoclonal antibody (68004-1-Ig)

    WB analysis of A431 using 68004-1-Ig

    A431 cells were subjected to SDS PAGE followed by western blot with 68004-1-Ig (ASNS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of rat pancreas tissue using ASNS Monoclonal antibody (68004-1-Ig)

    IHC staining of rat pancreas using 68004-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 68004-1-Ig (ASNS antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat pancreas tissue using ASNS Monoclonal antibody (68004-1-Ig)

    IHC staining of rat pancreas using 68004-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 68004-1-Ig (ASNS antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using ASNS Monoclonal antibody (68004-1-Ig)

    IF Staining of SH-SY5Y using 68004-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using ASNS antibody (68004-1-Ig, Clone: 1C3D3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

Tested Applications

Positive WB detected inK-562 cells, A431 cells, LNCaP cells, HeLa cells, HEK-293 cells, NIH/3T3 cells, 4T1 cells
Positive IHC detected inrat pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68004-1-Ig targets ASNS in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ASNS fusion protein Ag6817
Full Name asparagine synthetase
Calculated Molecular Weight 64 kDa
Observed Molecular Weight 55-64 kDa
GenBank Accession NumberBC014621
Gene Symbol ASNS
Gene ID (NCBI) 440
RRIDAB_2918751
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Asparagine synthetase [glutamine-hydrolyzing](ASNS) is also named as TS11. The asparagine synthetase gene, encoding the enzyme that catalyzes the synthesis of asparagine and glutamate using glutamine and aspartate, is a gene for which transcription is highly regulated by the nutritional status of the cell(PMID:15385533). ASNS has some isoforms with the MW of 55-64 kDa.

Protocols

Product Specific Protocols
WB protocol for ASNS antibody 68004-1-IgDownload protocol
IHC protocol for ASNS antibody 68004-1-IgDownload protocol
IF protocol for ASNS antibody 68004-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols