ASF/SF2 Monoclonal antibody, PBS Only

ASF/SF2 Monoclonal Antibody for WB, IP, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IP, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A6C5

Cat no : 66671-1-PBS

Synonyms

Alternative splicing factor 1, ASF, ASF 1, ASF/SF2, SF2, SF2p33, SFRS1, SRp30a, SRSF1

---

Validation Data Gallery

• 

  WB analysis using 66671-1-Ig (same clone as 66671-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66671-1-Ig (ASF/SF2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66671-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66671-1-Ig (same clone as 66671-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66671-1-Ig (ASF/SF2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66671-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HeLa using 66671-1-Ig (same clone as 66671-1-PBS)

  IP result of anti-ASF/SF2 (IP:66671-1-Ig, 5ug; Detection:66671-1-Ig 1:5000) with HeLa cells lysate 2200 ug. This data was developed using the same antibody clone with 66671-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 66671-1-Ig (same clone as 66671-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66671-1-Ig (ASF/SF2 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66671-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 66671-1-Ig (same clone as 66671-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66671-1-Ig (ASF/SF2 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66671-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 66671-1-Ig (same clone as 66671-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66671-1-Ig (ASF/SF2 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66671-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 66671-1-Ig (same clone as 66671-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66671-1-Ig (ASF/SF2 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66671-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 66671-1-Ig (same clone as 66671-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ASF/SF2 antibody (66671-1-Ig, Clone: 1A6C5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66671-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 66671-1-Ig (same clone as 66671-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66671-1-Ig (ASF/SF2 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66671-1-PBS in a different storage buffer formulation.

---

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66671-1-PBS targets ASF/SF2 in WB, IP, IF, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ASF/SF2 fusion protein Ag3626
• Full Name: splicing factor, arginine/serine-rich 1
• Calculated Molecular Weight: 248 aa, 28 kDa
• Observed Molecular Weight: 32 kDa, 28 kDa, 22 kDa
• GenBank Accession Number: BC010264
• Gene Symbol: ASF/SF2
• Gene ID (NCBI): 6426
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

SFRS1, also named as ASF, SF2, SF2P33, SFRS1 and ASF-1, belongs to the splicing factor SR family. It plays a role in preventing exon skipping, ensuring the accuracy of splicing and regulating alternative splicing. SFRS1 interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Isoform ASF-2 and isoform ASF-3 act as splicing repressors. This antibody can recognize all the 3 isoforms(28kd,33kd and 22kd) of SFRS1.