Various lysates were subjected to SDS PAGE followed by western blot with 66671-1-Ig (ASF/SF2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 66671-1-Ig (ASF/SF2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis using 66671-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 66671-1-Ig (ASF/SF2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 66671-1-Ig (ASF/SF2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 66671-1-Ig
IP result of anti-ASF/SF2 (IP:66671-1-Ig, 5ug; Detection:66671-1-Ig 1:5000) with HeLa cells lysate 2200 ug.
IP result of anti-ASF/SF2 (IP:66671-1-Ig, 5ug; Detection:66671-1-Ig 1:5000) with HeLa cells lysate 2200 ug.
IHC staining of human ovary tumor using 66671-1-Ig
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66671-1-Ig (ASF/SF2 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66671-1-Ig (ASF/SF2 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human gliomas using 66671-1-Ig
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66671-1-Ig (ASF/SF2 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66671-1-Ig (ASF/SF2 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human gliomas using 66671-1-Ig
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66671-1-Ig (ASF/SF2 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66671-1-Ig (ASF/SF2 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary tumor using 66671-1-Ig
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66671-1-Ig (ASF/SF2 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66671-1-Ig (ASF/SF2 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 66671-1-Ig
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ASF/SF2 antibody (66671-1-Ig, Clone: 1A6C5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ASF/SF2 antibody (66671-1-Ig, Clone: 1A6C5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).
IF Staining of HepG2 using 66671-1-Ig
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66671-1-Ig (ASF/SF2 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated Goat Anti-Mouse IgG(H+L).
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66671-1-Ig (ASF/SF2 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated Goat Anti-Mouse IgG(H+L).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
HeLa cells, LNCaP cells, HEK-293 cells, HepG2 cells, Jurkat cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells, RAW 264.7 cells
Positive IP detected in
HeLa cells
Positive IHC detected in
human ovary tumor tissue, human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
HepG2 cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:5000-1:50000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:200-1:800
Immunofluorescence (IF)/ICC
IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
SFRS1, also named as ASF, SF2, SF2P33, SFRS1 and ASF-1, belongs to the splicing factor SR family. It plays a role in preventing exon skipping, ensuring the accuracy of splicing and regulating alternative splicing. SFRS1 interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Isoform ASF-2 and isoform ASF-3 act as splicing repressors. This antibody can recognize all the 3 isoforms(28kd,33kd and 22kd) of SFRS1.
Various lysates were subjected to SDS PAGE followed by western blot with 66671-1-Ig (ASF/SF2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis using 66671-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 66671-1-Ig (ASF/SF2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human ovary tumor using 66671-1-Ig
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66671-1-Ig (ASF/SF2 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human gliomas using 66671-1-Ig
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66671-1-Ig (ASF/SF2 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human gliomas using 66671-1-Ig
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66671-1-Ig (ASF/SF2 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary tumor using 66671-1-Ig
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66671-1-Ig (ASF/SF2 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP Figures
IP experiment of HeLa using 66671-1-Ig
IP result of anti-ASF/SF2 (IP:66671-1-Ig, 5ug; Detection:66671-1-Ig 1:5000) with HeLa cells lysate 2200 ug.
IF/ICC Figures
IF Staining of HepG2 using 66671-1-Ig
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ASF/SF2 antibody (66671-1-Ig, Clone: 1A6C5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).
IF Staining of HepG2 using 66671-1-Ig
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66671-1-Ig (ASF/SF2 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated Goat Anti-Mouse IgG(H+L).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
At Proteintech, we pride ourselves on our antibody quality, customer service and transparency. As such, we are comparing our antibodies with other vendors, enabling easy identification and comparisons of key data to help you choose the suitable antibody for your needs.
We have selected the top cited antibodies from these vendors for you to compare.
Proteintech
ASF/SF2 Monoclonal antibody
Catalog Number
66671-1-Ig
Citations
2
Dilutions
WB : 1:5000-1:50000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:200-1:800 IF/ICC : 1:200-1:800
Applications
WB, IHC, IF/ICC, IP, ELISA
Reactivity
human, mouse, rat, pig
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
