WB analysis using 68145-1-Ig (same clone as 68145-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 68145-1-Ig (ARPC2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 68145-1-PBS in a different storage buffer formulation.
Various lysates were subjected to SDS PAGE followed by western blot with 68145-1-Ig (ARPC2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 68145-1-PBS in a different storage buffer formulation.
IHC staining of human stomach cancer using 68145-1-Ig (same clone as 68145-1-PBS)
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 68145-1-Ig (ARPC2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68145-1-PBS in a different storage buffer formulation.
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 68145-1-Ig (ARPC2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68145-1-PBS in a different storage buffer formulation.
IHC staining of human stomach cancer using 68145-1-Ig (same clone as 68145-1-PBS)
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 68145-1-Ig (ARPC2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68145-1-PBS in a different storage buffer formulation.
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 68145-1-Ig (ARPC2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68145-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 68145-1-Ig (same clone as 68145-1-PBS)
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using ARPC2 antibody (68145-1-Ig, Clone: 1D8E3 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68145-1-PBS in a different storage buffer formulation.
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using ARPC2 antibody (68145-1-Ig, Clone: 1D8E3 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68145-1-PBS in a different storage buffer formulation.
FC experiment of HeLa using 68145-1-Ig (same clone as 68145-1-PBS)
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ARPC2 (68145-1-Ig, Clone:1D8E3) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68145-1-PBS in a different storage buffer formulation.
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ARPC2 (68145-1-Ig, Clone:1D8E3) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68145-1-PBS in a different storage buffer formulation.
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68145-1-PBS targets ARPC2 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with Human, mouse, rat, pig, rabbit samples.
WB analysis using 68145-1-Ig (same clone as 68145-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 68145-1-Ig (ARPC2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 68145-1-PBS in a different storage buffer formulation.
IHC Figures
IHC staining of human stomach cancer using 68145-1-Ig (same clone as 68145-1-PBS)
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 68145-1-Ig (ARPC2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68145-1-PBS in a different storage buffer formulation.
IHC staining of human stomach cancer using 68145-1-Ig (same clone as 68145-1-PBS)
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 68145-1-Ig (ARPC2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68145-1-PBS in a different storage buffer formulation.
IF/ICC Figures
IF Staining of HeLa using 68145-1-Ig (same clone as 68145-1-PBS)
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using ARPC2 antibody (68145-1-Ig, Clone: 1D8E3 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68145-1-PBS in a different storage buffer formulation.
FC (INTRA) Figures
FC experiment of HeLa using 68145-1-Ig (same clone as 68145-1-PBS)
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ARPC2 (68145-1-Ig, Clone:1D8E3) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68145-1-PBS in a different storage buffer formulation.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
ARPC2 Monoclonal antibody
Catalog Number
68145-1-PBS
Citations
-
Dilutions
Applications
WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Reactivity
human, mouse, rat, pig, rabbit
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
