ARF4 Monoclonal antibody, PBS Only

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  WB analysis using 68204-1-Ig (same clone as 68204-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68204-1-Ig (ARF4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68204-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 68204-1-Ig (same clone as 68204-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 68204-1-Ig (ARF4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68204-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68204-1-Ig (same clone as 68204-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68204-1-Ig (ARF4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68204-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 68204-1-Ig (same clone as 68204-1-PBS)

  WB result of ARF4 antibody (68204-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ARF4 transfected HeLa cells. This data was developed using the same antibody clone with 68204-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68204-1-Ig (same clone as 68204-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68204-1-Ig (ARF4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68204-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68204-1-Ig (same clone as 68204-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68204-1-Ig (ARF4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68204-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 68204-1-Ig (same clone as 68204-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 68204-1-Ig (ARF4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68204-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 68204-1-Ig (same clone as 68204-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 68204-1-Ig (ARF4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68204-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 68204-1-Ig (same clone as 68204-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 68204-1-Ig (ARF4 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68204-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 68204-1-Ig (same clone as 68204-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 68204-1-Ig (ARF4 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68204-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 68204-1-Ig (same clone as 68204-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 68204-1-Ig (ARF4 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68204-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 68204-1-Ig (same clone as 68204-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 68204-1-Ig (ARF4 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68204-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 68204-1-Ig (same clone as 68204-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ARF4 antibody (68204-1-Ig, Clone: 2A4B8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68204-1-PBS in a different storage buffer formulation.

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  FC experiment of K-562 using 68204-1-Ig (same clone as 68204-1-PBS)

  1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human ARF4 (68204-1-Ig, Clone:2A4B8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (66360-3-Ig, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68204-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

ARF4 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Cat No. 68204-1-PBS

Clone No.2A4B8

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

ARF2, ADP-ribosylation factor 4, ADP ribosylation factor 4, 2A4B8

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Product Information

68204-1-PBS targets ARF4 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: ARF4 fusion protein Ag25522
• Full Name: ADP-ribosylation factor 4
• Calculated Molecular Weight: 180 aa, 21 kDa
• Observed Molecular Weight: 18 kDa
• GenBank Accession Number: BC003364
• Gene Symbol: ARF4
• Gene ID (NCBI): 378
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P18085
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

ARF4, also named as ARF2, belongs to the small GTPase superfamily and Arf family. ADP-ribosylation factors (ARFs) are members of the ARF family of GTP-binding proteins of the Ras superfamily, with 20 kDa protein size. ARFs bind and regulate GTP/GDP cycle by alternating between the active GTP-bound and inactive GDP-bound conformations. ARF family proteins are essential and ubiquitous in eukaryotes. Six highly conserved members of the family have been identified in mammalian cells. They function in vesicular traffic and actin remodelling and other bioprocesses in cells. ARF4 and ARF5 are class II ARFs. Their function are not fully described. (PMID: 7759471, PMID: 16042562). This antibody can bind ARFs for the close sequences.