ARF3 Polyclonal antibody, PBS Only

ARF3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 10800-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of mouse brain using 10800-1-AP (same clone as 10800-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10800-1-AP (ARF3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10800-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat brain using 10800-1-AP (same clone as 10800-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 10800-1-AP (ARF3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10800-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 10800-1-AP (same clone as 10800-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 10800-1-AP (ARF3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10800-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 10800-1-AP (same clone as 10800-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10800-1-AP (ARF3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10800-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Raji using 10800-1-AP (same clone as 10800-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 10800-1-AP (ARF3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10800-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse cerebellum using 10800-1-AP (same clone as 10800-1-PBS)

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 10800-1-AP (ARF3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10800-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 10800-1-AP (same clone as 10800-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 10800-1-AP (ARF3 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10800-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 10800-1-AP (same clone as 10800-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10800-1-AP (ARF3 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 10800-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 10800-1-AP (same clone as 10800-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10800-1-AP (ARF3 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 10800-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 10800-1-AP (same clone as 10800-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10800-1-AP (ARF3 antibody) at dilution of 1:10 (under 10x lens). This data was developed using the same antibody clone with 10800-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas using 10800-1-AP (same clone as 10800-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 10800-1-AP (ARF3 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 10800-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas using 10800-1-AP (same clone as 10800-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 10800-1-AP (ARF3 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 10800-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 10800-1-AP (same clone as 10800-1-PBS)

  Immunofluorescent analysis of HepG2 cells using 10800-1-AP (ARF3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10800-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 10800-1-AP (same clone as 10800-1-PBS)

  Immunofluorescent analysis of HepG2 cells using 10800-1-AP (ARF3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10800-1-PBS in a different storage buffer formulation.

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Product Information

10800-1-PBS targets ARF3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ARF3 fusion protein Ag1174
• Full Name: ADP-ribosylation factor 3
• Calculated Molecular Weight: 21 kDa
• Observed Molecular Weight: 18-20 kDa
• GenBank Accession Number: BC017565
• Gene Symbol: ARF3
• Gene ID (NCBI): 377
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

ADP-ribosylation factors (ARFs) are members of the ARF family of GTP-binding proteins of the Ras superfamily, with 20kda protein size. ARFs bind and regulate GTP/GDP cycle by alternating between the active GTP-bound and inactive GDP-bound conformations. ARF family proteins are essential and ubiquitous in eukaryotes. Six highly conserved members of the family have been identified in mammalian cells. They function in vesicular traffic and actin remodelling and other bioprocesses in cells. ARF3 is 95% homolouous to ARF1. Its function was not fully understood. (PMID: 7759471, PMID: 16042562). This antibody can bind ARFs for the close sequences.