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ARF3 Polyclonal antibody

ARF3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 10800-1-AP

Synonyms

ADP-ribosylation factor 3, ADP ribosylation factor 3

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using ARF3 Polyclonal antibody (10800-1-AP)

    WB analysis of mouse brain using 10800-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 10800-1-AP (ARF3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using ARF3 Polyclonal antibody (10800-1-AP)

    WB analysis of human brain using 10800-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 10800-1-AP (ARF3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse cerebellum tissue using ARF3 Polyclonal antibody (10800-1-AP)

    WB analysis of mouse cerebellum using 10800-1-AP

    mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 10800-1-AP (ARF3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using ARF3 Polyclonal antibody (10800-1-AP)

    WB analysis of HeLa using 10800-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 10800-1-AP (ARF3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using ARF3 Polyclonal antibody (10800-1-AP)

    WB analysis of HepG2 using 10800-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 10800-1-AP (ARF3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Raji cells using ARF3 Polyclonal antibody (10800-1-AP)

    WB analysis of Raji using 10800-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 10800-1-AP (ARF3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using ARF3 Polyclonal antibody (10800-1-AP)

    WB analysis of rat brain using 10800-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 10800-1-AP (ARF3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using ARF3 Polyclonal antibody (10800-1-AP)

    IHC staining of human kidney using 10800-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10800-1-AP (ARF3 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using ARF3 Polyclonal antibody (10800-1-AP)

    IHC staining of human kidney using 10800-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10800-1-AP (ARF3 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using ARF3 Polyclonal antibody (10800-1-AP)

    IHC staining of human pancreas using 10800-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 10800-1-AP (ARF3 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using ARF3 Polyclonal antibody (10800-1-AP)

    IHC staining of human pancreas using 10800-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 10800-1-AP (ARF3 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using ARF3 Polyclonal antibody (10800-1-AP)

    IHC staining of human pancreas cancer using 10800-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10800-1-AP (ARF3 antibody) at dilution of 1:10 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ARF3 Polyclonal antibody (10800-1-AP)

    IF Staining of HepG2 using 10800-1-AP

    Immunofluorescent analysis of HepG2 cells using 10800-1-AP (ARF3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ARF3 Polyclonal antibody (10800-1-AP)

    IF Staining of HepG2 using 10800-1-AP

    Immunofluorescent analysis of HepG2 cells using 10800-1-AP (ARF3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inmouse brain tissue, HeLa cells, HepG2 cells, human brain tissue, mouse cerebellum tissue, Raji cells, rat brain tissue
Positive IHC detected inhuman kidney tissue, human pancreas tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10800-1-AP targets ARF3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ARF3 fusion protein Ag1174
Full Name ADP-ribosylation factor 3
Calculated Molecular Weight 21 kDa
Observed Molecular Weight 18-20 kDa
GenBank Accession NumberBC017565
Gene Symbol ARF3
Gene ID (NCBI) 377
RRIDAB_2243128
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ADP-ribosylation factors (ARFs) are members of the ARF family of GTP-binding proteins of the Ras superfamily, with 20kda protein size. ARFs bind and regulate GTP/GDP cycle by alternating between the active GTP-bound and inactive GDP-bound conformations. ARF family proteins are essential and ubiquitous in eukaryotes. Six highly conserved members of the family have been identified in mammalian cells. They function in vesicular traffic and actin remodelling and other bioprocesses in cells. ARF3 is 95% homolouous to ARF1. Its function was not fully understood. (PMID: 7759471, PMID: 16042562). This antibody can bind ARFs for the close sequences.

Protocols

Product Specific Protocols
WB protocol for ARF3 antibody 10800-1-APDownload protocol
IHC protocol for ARF3 antibody 10800-1-APDownload protocol
IF protocol for ARF3 antibody 10800-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IHC

Development

PFN4 is required for manchette development and acrosome biogenesis during mouse spermiogenesis

Authors - Naila Umer
View Article